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目的探讨高脂饮食诱导的胰岛素抵抗大鼠糖代谢及抵抗素、脂联素水平的变化。方法采用大鼠扩展胰岛素钳夹和3-3H标记葡萄糖示踪技术,估计高脂诱导胰岛素抵抗(IR)大鼠肝糖及外周组织糖代谢的变化,同时采用酶免法检测血浆抵抗素及脂联素水平。结果高脂喂养组(HF组)大鼠基础血浆游离脂肪酸(FFA)水平明显增高。钳夹稳态时,HF组血浆胰岛素水平明显高于N组,差异有统计学意义(P<0.01),FFA也明显高于N组,差异有统计学意义(P<0.01)。HF组葡萄糖输注率(GIR)较N组减少38%,胰岛素介导的葡萄糖利用率(GRd)较N组亦明显减少21%。N组大鼠肝糖输出(HGP)明显抑制达71%,而在HF组胰岛素介导的HGP的抑制作用受到明显障碍(仅抑制30%)。钳夹结束时,N组血浆抵抗素水平与基础值相比明显降低,差异有统计学意义(P<0.05),而HF组明显高于N组(P<0.05)。两组血浆脂联素水平在钳夹后均明显下降差异有统计学意义(P<0.05)。结论高脂饮食诱导大鼠产生了外周和肝脏的IR。IR的形成可能与血浆FFA升高和在高胰岛素状态下较高的血浆抵抗素水平有关。
Objective To investigate the changes of glucose metabolism, resistin and adiponectin in rats with insulin resistance induced by high fat diet. Methods Expanded insulin clamp and 3-3H labeled glucose tracer were used to evaluate the changes of glycometabolism in peripheral blood and glycogen of rats with insulin resistance (IR) induced by high-fat diet. Plasma resistin and lipid Allianz levels. Results The level of plasma free fatty acid (FFA) in the HF group was significantly higher than that in the HF group. In steady state, the plasma insulin level in HF group was significantly higher than that in N group (P <0.01), and FFA was also significantly higher than that in N group (P <0.01). Glucose infusion rate (GIR) decreased by 38% in HF group compared with N group, and insulin-mediated glucose utilization (GRd) decreased by 21% compared with N group. The hepatic glucose output (HGP) was significantly inhibited by 71% in the N group, while insulin-mediated inhibition of HGP was significantly impaired (by only 30%) in the HF group. At the end of the clamp, the level of plasma resistin in N group was significantly lower than that of baseline (P <0.05), while the level in HF group was significantly higher than that in N group (P <0.05). Plasma adiponectin levels in both groups were significantly decreased after the clamp difference was statistically significant (P <0.05). Conclusion High fat diet induced peripheral IR and liver IR. The formation of IR may be associated with elevated plasma FFA and higher plasma resistin levels in hyperinsulinemic conditions.