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目的:研究马齿苋提取物(Portulaca oleracea extract,PLE)对大鼠心肌缺血再灌注损伤的保护作用并探讨其作用机制。方法:将动物随机分为假手术组、心肌缺血再灌注模型对照组、马齿苋提取物(10、20、40和80mg/kg)预处理组,术前4周开始灌胃给药,每天一次。分别测定各组大鼠心肌梗死面积;检测血清中总抗氧化能力(T-AOC)和谷草转氨酶(AST)、肌酸磷酸激酶(CK)、乳酸脱氢酶(LDH)、丙二醛(MDA)含量;测定心肌组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性、过氧化物酶(CAT)活性以及丙二醛(MDA)含量;采用HE染色方法观察心肌组织病理学改变和TUNEL染色观察心肌细胞凋亡状况。结果:与模型对照组相比,马齿苋提取物(40、80mg/kg)预处理组大鼠心肌梗死面积均显著降低;马齿苋提取物(20、40、80mg/kg)预处理组大鼠血清中AST、CK、LDH、MDA含量显著降低、T-AOC显著升高,心肌组织中SOD、GSH-Px、CAT活性显著升高,MDA含量显著降低,心肌组织病理学改变程度和细胞凋亡程度均明显较轻,并且该作用具有剂量依赖性。结论:马齿苋提取物能够有效减小心肌梗死面积、抑制心肌组织病理学改变和细胞凋亡,提示马齿苋提取物对心肌缺血再灌注损伤具有剂量依赖性的保护作用,该作用可能马齿苋提取物能够改善抗氧化酶活性、减轻自由基损伤有关。
Objective: To study the protective effect of Portulaca oleracea extract (PLE) on myocardial ischemia-reperfusion injury in rats and its mechanism. Methods: The animals were randomly divided into sham operation group, myocardial ischemia reperfusion model control group, purslane extract (10, 20, 40 and 80 mg / kg) pretreatment group, Once a day. The area of myocardial infarction in each group was measured. The levels of total antioxidant capacity (T-AOC), aspartate aminotransferase (AST), creatine phosphokinase (CK), lactate dehydrogenase (LDH), malondialdehyde (MDA) ), And the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), peroxidase (CAT) and malondialdehyde (MDA) Methods Myocardial histopathological changes and TUNEL staining were used to observe the apoptosis of cardiomyocytes. Results: Compared with the model control group, the area of myocardial infarction in the pretreated group of purslane extract (40, 80 mg / kg) was significantly decreased; the pretreatment group of purslane extract (20, 40, and 80 mg / The serum levels of AST, CK, LDH and MDA were significantly decreased, T-AOC was significantly increased, the activities of SOD, GSH-Px and CAT in myocardium were significantly increased, the content of MDA was significantly decreased, the degree of myocardial histopathological changes and cells The degree of apoptosis was significantly lighter, and the effect was dose-dependent. Conclusion: The purslane extract can effectively reduce the area of myocardial infarction, inhibit the pathological changes of myocardial tissue and apoptosis, suggesting that the purslane extract has a dose-dependent protective effect on myocardial ischemia-reperfusion injury, which may be Portulaca extract can improve antioxidant enzyme activity and reduce free radical damage.