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为研究乙酰化葡萄糖(Ac-G)对碱胁迫下小麦幼苗生长的影响,揭示其对碱胁迫下小麦幼苗生长的影响机制,以小麦品种山农129为材料,设置7个处理:W(蒸馏水)、CK(碱胁迫液)、T1(碱胁迫液+1 mmol/L Ac-G)、T2(碱胁迫液+2 mmol/L Ac-G)、T3(碱胁迫液+3 mmol/L Ac-G)、T4(碱胁迫液+4 mmol/L Ac-G)、T5(碱胁迫液+5 mmol/L Ac-G),测定了小麦幼苗的生理生态指标。结果表明,缓解胁迫的最适剂量为4 mmol/L乙酰化葡萄糖,碱胁迫液的p H值可降至7.44;随着乙酰葡萄糖剂量的增加,小麦幼苗株高、根长、根冠比、幼苗生物量、叶绿素a含量和叶绿素a/b呈现先增加后减小的变化趋势,在T4处理时达到最大值;随着乙酰化葡萄糖剂量的增加,幼苗叶片可溶性蛋白和可溶性糖的含量逐渐增加,最后趋于稳定;叶片中SOD和POD活性逐渐增强;在T4处理下,幼苗叶片的丙二醛含量和电导率达最小值。结果可为今后盐碱地改良提供重要的理论依据。
In order to study the effect of Ac-G on the growth of wheat seedlings under alkaline stress and to reveal the mechanism of its effect on the growth of wheat seedlings under alkaline stress, seven wheat cultivars, Shannong 129, were treated with W (distilled water ), CK (alkali stress solution), T1 (alkaline solution + 1 mmol / L Ac-G), T2 (alkaline solution + 2 mmol / L Ac-G) -G), T4 (alkaline stress solution +4 mmol / L Ac-G) and T5 (alkaline stress solution +5 mmol / L Ac-G) were used to determine the physiological and ecological indices of wheat seedlings. The results showed that the optimum dose of 4 mmol / L acetylated glucose was 4 mmol / L, the p H value of alkali stress solution could be reduced to 7.44. With the increase of the dosage of acetylglucose, the plant height, root length, The seedling biomass, chlorophyll a content and chlorophyll a / b showed the trend of increasing firstly and then decreasing, reaching the maximum value at T4 treatment. With the increase of the dosage of acetylated glucose, the content of soluble protein and soluble sugar in seedling leaves increased gradually , And finally stabilized. The activities of SOD and POD in leaves gradually increased. Under T4 treatment, the content of MDA and the conductivity of seedling leaves reached the minimum value. The results can provide important theoretical basis for the improvement of saline-alkali land in the future.