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目的探讨建立简捷、经济实用、稳定可靠的适于颈动脉粥样硬化性狭窄(CASS)的外科治疗研究的家兔CASS动物模型条件。方法新西兰白兔21只,采用3%过氧化氢液灌注动物的双侧颈总动脉,造成氧化应激损伤,然后以特定高脂饲料喂养动物不同时间。评价各组动物血管狭窄程度和病理改变特点。结果8周组和12周组中重度狭窄达到80%,个别血管出现闭塞。4周组轻度狭窄亦达到60%。病理检查证实高脂饲料喂养8周时,动脉的粥样硬化病理改变已属于纤维班快期。结论按本实验方法,氧化应激损伤后喂养8周的动物颈动脉狭窄程度和病理改变程度已符合CASS外科治疗实验研究需要。
Objective To establish a simple, economical, practical, stable and reliable CASS animal model for rabbits with surgical treatment of carotid atherosclerosis (CASS). Methods Twenty-one New Zealand white rabbits were infused with 3% hydrogen peroxide solution into bilateral carotid arteries to induce oxidative stress injury. Animals were then fed with specific high-fat diet for different times. The vascular stenosis and pathological changes in each group were evaluated. Results The severe stenosis reached 80% in 8-week group and 12-week group, and the occlusion of individual blood vessels occurred. 4 weeks group mild stenosis also reached 60%. Pathological examination confirmed that high-fat diet for 8 weeks, the arterial atherosclerosis pathological changes have been classified as fast fiber class. Conclusion According to the experimental method, the degree of carotid artery stenosis and the degree of pathological changes of animal fed 8 weeks after oxidative stress injury have met the need of CASS surgical treatment.