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对提高长期继代培养的籼稻花药愈伤组织再分化力的方法和技术进行了初步研究。结果表明继代培养基中添加的外源细胞分裂素配比以NAA 3mg/L +KT 1mg/L为好。用山梨醇部分取代蔗糖作碳源可大大提高籼稻花药愈伤组织的分化潜力。籼稻花药愈伤组织再分化前转移到添加ABA的预分化培养基进行预分化培养 ,可充分挖掘继代愈伤组织的再分化潜力 ,使愈伤组织的再分化率大大提高。通过长期继代培养的籼稻花药愈伤组织再生植株的白苗率与成熟胚愈伤组织再生植株白苗率相近。研究结果表明只要培养方法得当 ,籼稻花药愈伤组织经过长期继代培养后仍可保持较高的再分化力 ,因此以籼稻花药愈伤组织作遗传转化受体 ,在技术上是完全可行的。
The methods and techniques for improving the redistribution of anther callus of long-term subculture in indica rice were preliminarily studied. The results showed that the ratio of exogenous cytokinin added in subculture medium was better than NAA 3 mg / L + KT 1 mg / L. Partial replacement of sucrose with sorbitol as a carbon source can greatly improve the differentiation potential of indica anther callus. The anther callus of indica rice was transferred to the pre-differentiation medium supplemented with ABA before re-differentiation to fully excavate the potential of redifferentiation of subculture callus to greatly improve the rate of callus redifferentiation. The white seedling rate of regenerated plants of indica rice anther callus cultured by long-term subculture was similar to the white seedling rate of regenerated plants of mature embryo callus. The results showed that the indica anther callus could retain high re-differentiation force after long-term subculture, as long as the culture method was proper. Therefore, it is technically feasible to use indica anther callus as genetic transformation receptor.