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目的 了解敲除雄激素受体 (AR)后雄性小鼠的泌尿生殖系统表型。 方法 采用Cre lox技术 ,雌性Flox AR小鼠与雄性ACTB Cre小鼠交配 ,PCR方法检测子代小鼠尾巴的基因型 ,筛选出AR敲除小鼠 5只 ,5只AR未被敲除的小鼠作为对照。比较 2组小鼠的泌尿生殖系统表型 ,测量肛门生殖器距离、睾丸重量、血清睾酮及雌二醇浓度。 结果 AR敲除 (ARKO)组小鼠肛门生殖器距离为 (0 .5± 0 .1)cm ,对照组为 (1.1± 0 .1)cm。ARKO组前列腺、精囊、附睾及球海绵体肌均缺如 ,睾丸显著缩小 ,重量 (0 .0 0 6± 0 .0 0 1) g ;对照组前列腺、精囊、附睾及球海绵体肌发育正常 ,睾丸重量为 (0 .0 86± 0 .0 0 2 ) g。ARKO组血清睾酮浓度 (0 .0 5 6± 0 .0 4 5 )nmol/L ,雌二醇浓度 (1390 .1± 2 94 .3) pmol/L ,对照组睾酮浓度 (0 .84 3± 0 .736 )nmol/L ;雌二醇浓度 (786 .2± 15 0 .8) pmol/L。差异均有统计学意义 (P <0 .0 5 )。 结论 敲除雄激素受体后 ,小鼠前列腺、精囊、附睾及球海绵体肌缺如 ,睾丸缩小 ,雄激素水平降低 ,雌激素水平升高 ,提示AR在雄性小鼠泌尿生殖系统发生及雄激素水平调节中发挥重要作用。
Objective To understand the genitourinary phenotype of male mice after knockout of androgen receptor (AR). Methods Using Cre lox technique, female Flox AR mice were mated with male ACTB Cre mice. PCR was used to detect the genotypes of the offspring mouse tail. Five AR knockout mice were screened and five AR untreated Mouse as a control. The genito-urinary system phenotypes of two groups of mice were compared, anal genital distance, testicular weight, serum testosterone and estradiol concentrations were measured. Results The anal genital distance in AR knockout mice was (0.5 ± 0.1 cm) and that in the control group was (1.1 ± 0.1 cm). Prostate, seminal vesicle, epididymis and spheroids in ARKO group were all absent. Testicles were significantly reduced and weight was (0.060 ± 0.010) g. Prostate, seminal vesicle, epididymis and spheroids in control group were normal , Testicular weight (0. 86 ± 0. 0 0 2) g. Serum testosterone concentration (0. 05 6 ± 0. 04 5) nmol / L, estradiol concentration (1390 .1 ± 2 94. 3) pmol / L in ARKO group and testosterone concentration in control group 0. 736) nmol / L; estradiol concentration (786 .2 ± 15 0 .8) pmol / L. The differences were statistically significant (P <0.05). Conclusions After knockout of androgen receptor, the absence of prostate, seminal vesicles, epididymis and sphenoidis muscle of mice, the reduction of testes, the decrease of androgen levels, and the estrogen level, suggesting that AR occurs in the genitourinary system of male mice Hormone regulation plays an important role.