Currently, numerous automatic fabric defect detection algorithms have been proposed. Traditional machine vision algorithms that set separate parameters for different textures and defects rely on the manual design of corresponding features to complete the detection. To overcome the limitations of traditional algorithms, deep learning-based correlative algorithms can extract more complex image features and perform better in image classification and object detection. A pixel-level defect segmentation methodology using DeepLabv3+, a classical semantic segmentation network, is proposed in this paper. Based on ResNet-18,ResNet-50 and Mobilenetv2, three DeepLabv3+ networks are constructed, which are trained and tested from data sets produced by capturing or publicizing images. The experimental results show that the performance of three DeepLabv3+ networks is close to one another on the four indicators proposed（Precision, Recall, F1-score and Accuracy）, proving them to achieve defect detection and semantic segmentation, which provide new ideas and technical support for fabric defect detection.