目的建立HPLC测定鱼腥草芩蓝合剂中绿原酸、黄芩苷和黄芩素的含量。方法采用Kromasil-100 C18色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇-0.2%磷酸梯度洗脱,流速为1.0 m L·min-1,检测波长为322 nm,柱温为35℃。结果绿原酸、黄芩苷和黄芩素线性范围分别为0.022 4~1.12μg(R2=0.999 7),0.045 8~2.29μg(R2=0.999 5)和0.031 8~1.59μg(R2=0.999 9);3种成分的平均回收率分别为99.45%(RSD=1.4%),99.37%(RSD=2.0%)和97.90%(RSD=1.7%)。结论本方法快速简便,精密度高,稳定性和重复性好,可用于鱼腥草芩蓝合剂中绿原酸、黄芩苷和黄芩素3种成分的定量分析和药品质量控制。 OBJECTIVE To establish an HPLC method for the determination of chlorogenic acid, baicalin and baicalein in Yuxingcao Qinlan Mixture. Methods Kromasil-100 C18 column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase consisted of a gradient of methanol-0.2% phosphoric acid. The flow rate was 1.0 m L · min-1. The detection wavelength was set at 322 nm and the column temperature was 35 ℃. Results The linear ranges of chlorogenic acid, baicalin and baicalein were 0.022 4-1.12 μg (R2 = 0.999 7), 0.045 8-2.29 μg (R2 = 0.999 5) and 0.031 8-1.59 μg (R2 = 0.999 9), respectively. The average recoveries of the three components were 99.45% (RSD = 1.4%), 99.37% (RSD = 2.0%) and 97.90% (RSD = 1.7%), respectively. Conclusion The method is rapid, simple, accurate, stable and reproducible. It can be used for quantitative analysis and quality control of chlorogenic acid, baicalin and baicalein in Yuxingcao Qinlan Mixture.