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目的 探讨孕妇外周血及胎盘组织中胎儿有核红细胞 (nucleated red blood cell,NRBC)的出现频率与胎儿生长受限 (fetal growth restriction,FGR)的关系。 方法 对 2 0例孕 2 8～36周 ,年龄 2 1～ 30岁 (包括 9例 FGR)的孕妇外周血进行不连续密度梯度离心 ,对分离后的细胞进行制片 ,显微镜下进行 NRBC计数 ,比较组间差异 ;追随至终止妊娠时 ,对胎盘组织进行切片 ,显微镜下进行 NRBC计数 ,比较组间差异 ;显微操作法获取 5例单个 NRBC行引物延伸预扩增 (PEP)和聚合酶链反应 (PCR) ,验证其胎儿细胞来源。 结果 9例 FGR妊娠妇女外周血中 NRBC数目从 12个 / 7ml～ 4 0个 / 7ml不等 ,平均为 2 2 .6个 / 7m l。而同孕龄正常妊娠妇女外周血中 NRBC数目从 0个 / 7ml～ 10个 / 7ml不等 ,平均为 5 .4个 / 7ml,两者间差异有极显著性 (P<0 .0 0 1) ;FGR妊娠妇女胎盘绒毛间质血管中 NRBC数目从 2个 / 2 0 HP～ 5个 / 2 0 HP不等 ,平均为 2 .8个 / 2 0 HP。而正常妊娠妇女则从 0个 / 2 0 HP～ 2个 / 2 0 HP不等 ,平均为 0 .6个 / 2 0 HP,两者间差异有显著性 (P<0 .0 5 )。 结论 FGR妊娠妇女其外周血与胎盘组织中 NRBC数目明显升高
Objective To investigate the relationship between the frequency of fetal nucleated red blood cells (NRBC) and fetal growth restriction (FGR) in peripheral blood and placenta of pregnant women. Methods 20 pregnant women with gestational age from 2 to 36 weeks and 21 to 30 years of age (including 9 cases of FGR) were subjected to discontinuous density gradient centrifugation. The isolated cells were prepared and counted under a microscope. The placental tissues were sectioned and the count of NRBC was counted under the microscope to compare the differences between the groups. Five cases of single pre-extension extension of pre-amplification (PEP) and PCR Response (PCR) to verify its fetal cell origin. Results The number of NRBC in peripheral blood of 9 pregnant women with FGR ranged from 12 / 7ml to 40 / 7ml, with an average of 22.6 / 7ml. However, the number of NRBC in peripheral blood of pregnant women of the same gestational age ranged from 0 / 7ml to 10 / 7ml with an average of 5.4 / 7ml, the difference was significant (P <0. ). The number of NRBC in placental villi interstitial blood vessels of FGR pregnant women varied from 2/20 HP to 5/20 HP with an average of 2.8 / 20 HP. The normal pregnant women from 0/20 HP ~ 2/20 HP ranging from an average of 0.6 / 20 HP, the difference between the two was significant (P <0.05). Conclusion The number of NRBC in peripheral blood and placenta of FGR pregnant women is significantly higher than that in normal pregnancy