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为研究高氟对新西兰兔组织抗氧化酶和一氧化氮合酶(NOS)的影响以及硒的拮抗作用,将20只健康清洁级雄性新西兰兔随机分为加氟组(含氟100mg/L)、加硒组(含硒1mg/L)、加氟加硒组(含氟100mg/L、硒1mg/L)和对照组(去离子水),每组5只,采用自由饮水方式染毒,连续染毒6个月。测定血清氟含量和超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、总一氧化氮合酶(NOS)、诱导型一氧化氮合酶(iNOS)活力以及丙二醛(MDA)含量。结果显示,各组体重间比较,差异无统计学意义(P>0.05)。与对照组相比,加氟组和加氟加硒组的血清氟含量较高,差异有统计学意义(P<0.01)。与加氟组相比,加硒组和加氟加硒组血清氟含量较低,差异有统计学意义(P<0.05或P<0.01)。高氟可引起心肌、肝脏组织中SOD、GSH-Px活力下降、MDA含量升高、总NOS和iNOS活力升高,差异均有统计学意义(P<0.05或P<0.01)。染硒可使高氟新西兰兔上述各项指标有所改善(P<0.05或P<0.01)。表明氟中毒可导致组织抗氧化酶活力降低,脂质过氧化物含量增加,适量硒对其有拮抗作用。
In order to study the effect of fluoride on antioxidant enzymes and nitric oxide synthase (NOS) and selenium antagonism in New Zealand rabbits, twenty healthy male New Zealand white rabbits were randomly divided into fluoride group (containing 100 mg / L fluoride) , Selenium group (1mg / L), fluoride plus selenium group (fluoride 100mg / L, selenium 1mg / L) and control group (deionized water) Continuous exposure for 6 months. The levels of serum fluoride and activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), total nitric oxide synthase (NOS) and inducible nitric oxide synthase (iNOS) Malondialdehyde (MDA) content. The results showed that there was no significant difference between groups in body weight (P> 0.05). Compared with the control group, fluoride levels in the fluoride group and fluoride plus selenium group were significantly higher (P <0.01). Compared with fluoride group, serum fluoride content in selenium group and fluoride plus selenium group was lower (P <0.05 or P <0.01). High fluoride could cause the activity of SOD and GSH-Px in myocardium and liver to decrease, the content of MDA increased, the activity of total NOS and iNOS increased, the differences were statistically significant (P <0.05 or P <0.01). Selenium exposure improved the above indices in high-fluoride New Zealand rabbits (P <0.05 or P <0.01). Fluorosis can lead to tissue antioxidant activity decreased, lipid peroxidation increased, the amount of selenium antagonistic effect.