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建立了一种用超声波辅助萃取-高效液相法测定烟草中β-D-吡喃葡萄糖-3-氧代-α-紫罗兰醇苷含量的新方法。以甲醇为萃取溶剂,超声萃取条件经过正交实验优化,优化后的条件为料液比1:40(m/V,g/mL)、萃取功率160W,萃取时间20 min。所得萃取液经大孔吸附树脂柱层析法分离后,用Waters SunFireC18(150 mm×4.6 mm,5μm)色谱柱分离,紫外检测器(波长为243nm)检测,流动相为V(乙腈):V(水)=20:80;流速1 mL/min。β-D-吡喃葡萄糖-3-氧代-α-紫罗兰醇苷在0.01～1 mg/mL范围内线性关系良好,相关系数为0.9994,相对标准偏差为1.8%,检出限为0.05μg/mL,平均回收率为87.80%。该方法适用于β-D-吡喃葡萄糖-3-氧代-α-紫罗兰醇苷的定量分析。
A new method for the determination of β-D-glucopyranosyl-3-oxo-α-ionol glycoside in tobacco by ultrasonic-assisted extraction and high performance liquid chromatography was established. Using methanol as extraction solvent, the conditions of ultrasonic extraction were optimized by orthogonal experiments. The optimal conditions were as follows: solid-liquid ratio 1:40 (m / V, g / mL), extraction power 160 W, extraction time 20 min. The obtained extract was separated by macroporous resin column chromatography and then separated on a Waters SunFire C18 (150 mm × 4.6 mm, 5 μm) column with an ultraviolet detector (wavelength of 243 nm). The mobile phase consisted of V (acetonitrile): V (Water) = 20:80; flow rate 1 mL / min. β-D-glucopyranosyl-3-oxo-α-ionolide had a good linearity in the range of 0.01-1 mg / mL with a correlation coefficient of 0.9994 and a relative standard deviation of 1.8% with a detection limit of 0.05 μg / mL, the average recovery was 87.80%. This method is suitable for the quantitative analysis of β-D-glucopyranosyl-3-oxo-α-ionolide.