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目的 探讨大鼠局灶性脑缺血再灌注后血浆血小板活化因子(PAF)、PAF受体基因表达的变化。方法 线栓法建立大鼠大脑中动脉闭塞(MCAO)及再通模型,应用RT PCR技术检测MCAO及再通后缺血半暗带皮质PAF受体基因表达,同时用ELISA法检测对应血浆PAF值。结果 单纯脑缺血24 h时缺血区皮质PAF受体mRNA含量并无显著变化,密度比值为0.98±0.13,再灌注6 h明显降低(0.63±0.08),24 h最低(0.44±0.06),随后逐渐回升。对应时相血浆PAF值单纯脑缺血组为(848±80)(pg/ml),再灌注12 h为最高,48 h降至对照组水平(568±45)(pg/ml)。结论 脑缺血再灌注可致PAF受体基因表达异常,推测与内源性PAF水平升高有关。
Objective To investigate the changes of plasma platelet activating factor (PAF) and PAF receptor gene expression after focal cerebral ischemia / reperfusion in rats. Methods The rat model of middle cerebral artery occlusion (MCAO) and recanalization was established by the method of thread plug. The expression of PAF receptor gene in the cortex of MCAO and cataracts in the ischemic penumbra was detected by reverse transcription polymerase chain reaction (RT PCR) . Results The expression of PAF receptor mRNA in ischemic cortex did not change significantly at 24 h after ischemia, the density ratio was 0.98 ± 0.13, significantly decreased 6 h after reperfusion (0.63 ± 0.08), and lowest at 24 h (0.44 ± 0.06) Then gradually picked up. PAF in corresponding phase and phase group was (848 ± 80) pg / ml, highest at 12 h after reperfusion, and decreased to 568 ± 45 pg / ml in 48 h. Conclusions Cerebral ischemia / reperfusion can cause abnormal gene expression of PAF receptor, which is presumed to be related to the increase of endogenous PAF level.