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目的:建立一种可测定首乌丸中补骨脂素和异补骨脂素的RP-HPLC方法。方法:采用Shim-packVP-ODS(250mm×4.6mm,5μm)为色谱柱;流动相为甲醇-水(45∶55);检测波长为246nm;流速为1.0mL/min;柱温为40℃;峰面积外标法定量。结果:此法补骨脂素在9.10×10-4~0.2742μg范围内呈良好的线性关系,(r=0.99999),平均回收率为97.8%,RSD为2.27%;异补骨脂素在9.90×10-4~0.2970μg范围内呈良好的线性关系,(r=0.99999),平均回收率为100.4%,RSD 2.44%。结论:此方法简便、快速、准确可靠,可作为该制剂的质控方法。
Objective: To establish a RP-HPLC method for the determination of psoralen and isopsoralen in Shouwu pill. Methods: Shim-pack VP-ODS (250mm × 4.6mm, 5μm) was used as the column. The mobile phase was methanol-water (45:55). The detection wavelength was 246nm. The flow rate was 1.0mL / Peak area of external standard method. Results: The psoralen showed a good linear relationship in the range of 9.10 × 10-4 ~ 0.2742μg (r = 0.99999), the average recovery was 97.8%, the RSD was 2.27%, and the isopsoralen was at 9.90 × 10-4 ~ 0.2970μg, and the average recovery was 100.4% and RSD 2.44%, respectively (r = 0.99999). Conclusion: This method is simple, rapid, accurate and reliable and can be used as a quality control method for this preparation.