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Angucycline类抗生素是一类具有多种活性的化合物。现有研究表明angucycline合成过程的环化酶可以作为分子标签探测微生物产angucycline的潜力,指导新angucycline抗生素的开发。基于对环化酶的特异扩增和分析,本研究对来自中国南海17个底泥样品的angucycline基因资源进行了初步调查,结果显示10个底泥中存在新的angucycline抗生素资源,其中一种新的angucycline抗生素资源还同时存在于花坛土壤样品和放线菌Streptomyces sp.中。这表明中国南海底泥普遍存在着新的angucycline抗生素资源,部分angucycline抗生素资源可以通过普通的土壤样品和放线菌进行挖掘,从而避免复杂的取样过程。本研究为利用培养方法和不依赖于培养的宏基因组方法开发angucycline抗生素奠定了基础。
Angucycline antibiotics are a class of compounds that have multiple activities. Existing studies indicate that the enzymatic cyclase of angucycline synthesis can be used as a molecular marker to probe the potential of angucycline-producing microorganisms to guide the development of new angucycline antibiotics. Based on the specific amplification and analysis of cyclase, a preliminary investigation of angucycline gene resources from 17 sediment samples from the South China Sea showed that there were new angucycline antibiotics resources in 10 sediments, of which a new The angucycline antibiotic resource is also present in flowerbed soil samples and in actinomycetes Streptomyces sp. This suggests that new angucycline antibiotic resources are common in the sediments of South China Sea and that some angucycline antibiotic resources can be excavated using ordinary soil samples and actinomycetes to avoid complicated sampling. This study laid the foundation for the development of angucycline antibiotics using culture methods and metagenomic methods that are independent of culture.