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目的 HPLC法测定复方羊角片中白芷的含量,为该中成药的质量控制提供了客观的定量方法。方法以十八烷基硅烷键合硅胶为填充剂;以甲醇:水(55:45)为流动相;超声提取;检测波长为300nm。结果欧前胡素浓度在0.0396~0.1980 mg/ml范围内,线性关系良好(r=0.99959),平均回收率99.80%;RSD 0.37%,小于2.0%。结论采用HPLC法测定复方羊角片中白芷的含量,此方法分辨率高、速度快、专属性强、重复性好,可控制和提高复方羊角片质量。
OBJECTIVE To determine the content of Angelica dahuricae in Compound Gingko Tablets by HPLC and to provide an objective and quantitative method for the quality control of this proprietary Chinese medicine. Methods octadecylsilane bonded silica as a filler; methanol: water (55:45) as mobile phase; ultrasonic extraction; detection wavelength of 300nm. Results The concentration of Imperatorin in the range of 0.0396 ~ 0.1980 mg / ml had a good linear relationship (r = 0.99959) with an average recovery of 99.80%; RSD 0.37% and less than 2.0%. Conclusion HPLC method was used to determine the content of Angelica dahuricae. The method has the advantages of high resolution, fast speed, high specificity and good repeatability, which can control and improve the quality of the compound.