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目的:建立白灵片处方中人参皂苷Rg1、Rb1的含量测定方法。方法:比较HPLC-ELSD与HPLC法,采用HPLC-ELSD梯度洗脱的方法进行色谱分离。色谱柱:大连依利特ODS柱;流动相为乙腈-水;梯度洗脱;漂移管温度95℃。结果:人参皂苷Rgl在0.28~4.27μg范围内呈现良好线性关系(r=0.9992);人参皂苷Rb1在0.30~4.54μg范围内呈现良好线性关系(r=0.9998),平均回收率分别为102.5%和101.8%,RSD分别为2.8%和2.3%。结论:该测定方法操作简便、结果准确,可用于白灵片质量控制。
Objective: To establish a method for the determination of ginsenosides Rg1 and Rb1 in Bai Ling tablets. Methods: HPLC-ELSD and HPLC methods were compared using HPLC-ELSD gradient elution. Column: Dalian Elite ODS column; mobile phase acetonitrile-water; gradient elution; drift tube temperature 95°C. RESULTS: The ginsenoside Rgl showed a good linear relationship in the range of 0.28-4.27 g (r=0.9992). The ginsenoside Rb1 showed a good linear relationship (r=0.9998) in the range of 0.30 to 4.54 μg. The average recovery was 102.5% and 101.8%, RSD were 2.8% and 2.3% respectively. Conclusion: The method is simple and accurate, and can be used for quality control of Bailing tablets.