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目的 :探讨树对丙型肝炎病毒 (HCV)的易感性。方法 :对来自云南的树接种含 HCV的病人血清 ,然后用 PCR方法检测树血清的 HCV RNA,用反向被动血凝法测定抗 -HCV抗体 ,用原位 PCR方法检测树肝细胞内的 HCV RNA。结果 :34 .8%的树 (8/ 2 3)在接种 HCV后 ,血清中检出 HCV RNA,对 4例抗 HCV阳性和谷丙转氨酶升高的树的肝组织进行原位 PCR检测 ,结果在 1例肝细胞中检出 HCV RNA。此外 ,30 .4 %的树 (7/ 2 3)在接种后出现抗 -HCV抗体。部分阳性树均有不同程度的谷丙转氨酶异常升高及肝组织病理性炎症反应。对照组动物 (2只 )从未出现 HCV RNA及抗 -HCV抗体 ,但其中一只树曾有一过性转氨酶升高。结论 :树可以感染 HCV。
Objective: To investigate the susceptibility of tree shrews to hepatitis C virus (HCV). Methods: The sera of patients with HCV were inoculated into the tree shrews from Yunnan. The HCV RNA of the tree shrews was detected by PCR. The anti-HCV antibodies were determined by reverse passive hemagglutination. The tree shrews were detected by in situ PCR Within the HCV RNA. Results: 34.8% of tree shrews (8/2 3) detected HCV RNA in serum after inoculation of HCV, and in situ PCR of 4 liver tissues of tree shrews with anti-HCV positive and elevated alanine aminotransferases As a result, HCV RNA was detected in one case of hepatocytes. In addition, 30.4% of trees (7/2 3) developed anti-HCV antibodies after vaccination. Some positive tree shrews have varying degrees of alanine aminotransferase abnormalities and pathological liver inflammation. Control animals (2 animals) never developed HCV RNA and anti-HCV antibodies, but one of the tree shrews had transient transaminase elevations. Conclusion: Tree shrew can infect HCV.