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莱氏野村菌(Nomuraea rileyi)cq菌株为昆虫致病性真菌,能感染家蚕发生绿僵病。显微镜下观察菌株体外培养产生的分生孢子呈卵圆形或椭圆形,大小为3.2～4.6μm×2.5～3.2μm,分生孢子梗单生,具有3～4个轮状分支,每个分支着生2～3个瓶梗,瓶梗基部膨大,在瓶梗顶端尖处着生分生孢子。在感染莱氏野村菌的家蚕幼虫血液中可观察到菌株产生的大量豆荚状、酵母状芽生孢子,病蚕血液呈浑浊的乳白色,体表出现褐色病斑,反应迟缓,尸体逐渐僵化,被鲜绿色的孢子粉覆盖。另发现该菌株在感染家蚕幼虫的体表和血液中能产生菱形晶体,同时菌株在体外培养基上培养时也观察到大量的正八面体菱形晶体,且改变培养温度和pH值,晶体产生的时间延长,形状和大小也会发生变化,出现长方形、正方形六面体形状及不规则形状晶体。推测莱氏野村菌cq菌株产生的晶体可能是有利于菌株感染家蚕的一种次生代谢产物。
Nomuraea rileyi cq strain is an insect-pathogenic fungus that infects silkworms. Microscopically, the conidia produced by in vitro culture were oval or oval with the size of 3.2 ~ 4.6μm × 2.5 ~ 3.2μm. Conidiophores were simple and had 3 ~ 4 rotifer branches. Each branch With students 2 to 3 bottles of stems, ampulla inflated base, at the tip of the bottle stems with raw conidia. In the larval Rhizoctonia solani larval blood can be observed in large numbers of strains produced pods, yeast-like bud spores, silkworm blood turbid milky white, body surface brown spot, slow response, the body gradually rigid, fresh Green spore powder covered. In addition, a large number of rhombohedral octahedron crystals were also observed when the strain was cultured on in vitro culture medium, and the culture temperature and pH, the time of crystal production Extend, shape and size will change, there rectangular, square hexahedral shape and irregular shape of the crystal. It is speculated that the crystal produced by ct strain of Rhizoctonia solani may be a secondary metabolite that is beneficial to the infection of silkworm strains.