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目的研究儿童沙门菌整合子分布及其与产β-内酰胺酶细菌耐药性的关系。方法采用MIC法或纸片扩散法进行沙门菌药敏培养及表型鉴定;PCR方法检测沙门菌整合子和ESBLs耐药基因。结果 80株沙门菌检测出ESBLs耐药表型阳性率为21.3%(17/80)。ESBLs耐药基因阳性率为17.5%(14/80),其中CTX-M、TEM、CTX-M/TEM耐药基因阳性株分别为6株、6株和2株,未检测到SHV耐药基因。80株沙门菌检测出整合子阳性率为11.3%(9/80),其中8株为Ⅰ类整合子,1株为Ⅱ类整合子,未检出Ⅲ类整合子。1株Ⅰ类整合子阳性株表现对全部β-内酰胺类抗生素敏感,其余为对氨苄西林和/或氨苄西林/舒巴坦和/或头孢噻肟耐药。结论杭州地区腹泻患儿沙门菌对β-内酰胺抗生素耐药主要是CTX-M型ESBLs耐药基因;整合子在腹泻患儿产ESBLs沙门菌中的分布明显低于非产ESBLs菌株,与ESBLs耐药表型及ESBLs耐药基因之间无明显关系,且与β-内酰胺抗生素耐药性无明显关系。
Objective To study the distribution of Salmonella integron in children and its relationship with β-lactamase-producing bacteria. Methods Salmonella susceptibility culture and phenotype identification were performed by MIC method or disk diffusion method. Salmonella integrants and ESBLs-resistant genes were detected by PCR. Results The positive rate of ESBLs-resistant phenotype detected by 80 Salmonella strains was 21.3% (17/80). The positive rate of ESBLs-resistant genes was 17.5% (14/80). The positive rates of CTX-M, TEM and CTX-M / TEM were 6, 6 and 2 respectively. . The positive rate of integron detected by 80 strains of Salmonella was 11.3% (9/80), of which 8 were class Ⅰ integron and 1 was class Ⅱ integron. No class Ⅲ integron was detected. One strain of class I integron positive was sensitive to all beta-lactam antibiotics and the remainder was resistant to ampicillin and / or ampicillin / sulbactam and / or cefotaxime. Conclusion The resistance to β-lactam antibiotics in children with diarrhea in Hangzhou is mainly CTX-M type ESBLs-resistant gene. The distribution of integron in Salmonella producing ESBLs in children with diarrhea is obviously lower than that in non-ESBLs-producing ESBLs strains, There was no significant relationship between drug resistance phenotype and ESBLs resistance genes, and there was no significant relationship between them and β-lactam antibiotic resistance.