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目的探讨不同浓度非促分裂人酸性成纤维细胞生长因子(nmhaFGF)对肾脏缺血-再灌注(I-R)损伤后内皮素1(ET-1)和一氧化氮(NO)含量的影响。方法成年SD大鼠35只随机分为假手术(切除左肾,暴露右肾)(SA)组和4个模型组(切除左肾,右肾动静脉夹闭60min)。建模后5min经舌静脉注射nmhaFGF10μg/kg(A组)、20μg/kg(B组)、40μg/kg(C组)或生理盐水1.5ml(D组)。术后24h处死,放射免疫及生化方法检测肾组织和血浆ET-1和NO含量。结果B组皮质和外髓ET-1含量明显低于D组(P<0.05),C组肾皮质、外髓、内髓和血清中ET-1含量都明显低于D组(P<0.01);B、C组肾组织中NO含量明显高于D组(P<0.05)。结论nmhaFGF可以通过降低ET-1和升高NO水平减轻大鼠肾脏I-R损伤。
Objective To investigate the effects of different concentrations of non-mitogenic human acidic fibroblast growth factor (nmhaFGF) on the content of ET-1 and NO after renal ischemia-reperfusion (I-R) injury. Methods Thirty - five adult Sprague - Dawley rats were randomly divided into 4 groups: sham operation (excised left kidney and exposed right kidney) (SA group) and 4 model groups (excised left kidney and right renal artery and vein occluded for 60 minutes). Five minutes after modeling, the rats were injected intravenously with nmhaFGF 10μg / kg (group A), 20μg / kg (group B), 40μg / kg (group C) or normal saline 1.5ml (group D). After 24h of operation, radioimmunoassay and biochemical methods were used to detect the contents of ET-1 and NO in renal tissue and plasma. Results The content of ET-1 in cortical and extraneous pulp of group B was significantly lower than that in group D (P <0.05). The content of ET-1 in renal cortex, extramedullary, medulla and serum in group C was significantly lower than that in group D (P <0.01) The content of NO in group B and C was significantly higher than that in group D (P <0.05). Conclusion nmhaFGF can reduce rat renal I-R injury by decreasing ET-1 and increasing NO levels.