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通过配体交换法,在Au NPs表面分别引入羟基(-OH),羧基(-COOH)和甲基(-CH3),制备了3种表面修饰官能团的金纳米粒:Au-OH NPs,Au-COOH NPs和Au-CH3NPs,其平均粒径为(15.6±3.2)nm,ζ电位均为负值。MTT法对比研究表面修饰和未修饰的Au NPs与He La细胞和MCG-803细胞作用后的细胞存活率,当浓度达到197 ng·m L-1时,表现出低细胞毒性,且顺序为:Au NPs>Au-CH3NPs>Au-COOH NPs≈Au-OH NPs。细胞周期研究结果发现,表面未修饰的Au NPs对细胞G2/M期活动有一定的阻滞作用。单个活细胞显微拉曼光谱原位对比研究表面修饰和未修饰的Au NPs与He La细胞的作用,结果表明:未修饰的Au NPs和Au-CH3NPs与细胞作用的主靶点可能为DNA骨架、碱基和细胞磷脂膜的极性头部,而Au-COOH NPs与Au-OH NPs对这些位点作用轻微。本研究为解释表面修饰-COOH和-OH官能团可降低Au NPs细胞毒性提供了研究证据。
Gold nanoparticles (Au-OH NPs, Au-OH NPs) with surface-modified functional groups were prepared by the ligand exchange method by introducing hydroxyl group (-OH), carboxyl group (-COOH) and methyl group (-CH3) COOH NPs and Au-CH3NPs, the average particle size of (15.6 ± 3.2) nm, zeta potential were negative. MTT assay showed that the cell viability of surface-modified and non-modified Au NPs treated with He La cells and MCG-803 cells was low cytotoxicity when the concentration reached 197 ng · m L-1, and the order was: Au NPs> Au-CH3NPs> Au-COOH NPs≈Au-OH NPs. Cell cycle study found that the surface of the unmodified Au NPs on cell G2 / M phase activity has a certain block effect. The single Raman spectra of living cells were used to study the effects of surface-modified and unmodified Au NPs on HeLa cells in situ. The results showed that the main targets of the unmodified Au NPs and Au-CH3NPs on the cells may be the DNA backbone , Alkaloids, and cellular phospholipid membranes, whereas Au-COOH NPs and Au-OH NPs did little to these sites. This study provides evidence to explain that surface modification of -COOH and -OH functional groups can reduce the cytotoxicity of Au NPs.