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目的对2012年-2014年珠海市流行的Ⅰ型登革病毒(DV)进行包膜蛋白基因序列测定,分析病毒可能的来源。方法采集2012年-2014年珠海登革热流行期间登革热疑似和临床诊断病例的急性期血清696份,采用荧光RT-PCR检测登革病毒核酸,选取46份DVⅠ型核酸阳性标本,用荧光RT-PCR扩增包膜蛋白基因并进行测序分型。结果荧光RT-PCR检测DV通用型核酸阳性为152例,阳性率为21.84%(152/696),其中DVⅠ型阳性为148例;选取的46份标本全部成功分型。DVⅠ型碱基同源性为89.34%~99.77%,其同源性与2014年中国广州地区、2013年中国中山地区和2011年印度的DVⅠ型流行株接近。结论 2012年-2014年珠海市DV流行主要以Ⅰ型为主,流行方式属于周边地市或东南亚国家输入性病例引起的本地暴发。
Objective To analyze the sequence of envelope protein gene of epidemic type Ⅰ dengue virus (DV) in Zhuhai from 2012 to 2014 and analyze the possible origin of the virus. Methods A total of 696 serum samples were collected during the epidemic period of dengue in Zhuhai from 2012 to 2014. The serum samples of dengue virus were detected by RT-PCR. Forty-six DVⅠ-positive samples were obtained by fluorescence RT-PCR Envelope protein gene and sequencing typing. Results The positive rate of DV universal nucleic acid detected by RT-PCR was 152 cases (21.84%, 152/696), of which 148 were positive for DVⅠ. All the 46 samples were successfully typed. The homology of DVⅠbase was 89.34% -99.77%, which was close to that of DV Ⅰ in 2014 in Guangzhou, China, 2013 in Zhongshan, China and India in 2011. Conclusions From 2012 to 2014, the epidemic of DV in Zhuhai City was dominated by type I, and the epidemic pattern belonged to the local outbreak caused by imported cases in the surrounding cities or Southeast Asian countries.