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目的观察怀槐异黄酮对雌激素诱导小鼠肝损伤的保护作用并探讨其作用机制。方法选择BALB/c孕鼠随机分为正常组、溶剂组、肝损伤模型组和大、中、小剂量异黄酮给药组,每组10只,给药组从受孕第7天开始分别灌胃异黄酮,每天1次,共7 d。除正常组外,各组从妊娠10 d起连续4 d皮下注射17-α-乙炔雌二醇1.0μg/g体重。常规检测孕鼠血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)活性水平和肝脏组织病理。结果给异黄酮的孕鼠血清ALT、AST活性水平显著下降,与肝损伤模型孕鼠比较差异有统计学意义(P<0.05),其肝脏组织病理学结果也证实给药孕鼠肝脏病理明显改善。结论怀槐异黄酮对雌激素诱导的小鼠肝损伤有显著保护作用。
Objective To observe the protective effect of Huaihuai isoflavones on estrogen-induced liver injury in mice and to explore its mechanism. Methods BALB / c pregnant mice were randomly divided into normal group, solvent group, liver injury model group and large, medium and small doses of isoflavone administration group, with 10 rats in each group. The rats in treatment group were given gavage Isoflavone, 1 day, a total of 7 d. Except the normal group, 17-α-ethinyl estradiol 1.0 μg / g body weight was injected subcutaneously every 4 days from the 10th day of pregnancy in each group. Routine detection of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) activity levels and liver histopathology. Results The levels of serum ALT and AST in pregnant rats with isoflavone decreased significantly compared with those in the liver injury model (P <0.05), and the liver histopathological results also showed that the liver pathology of the pregnant rats administered with isoflavone significantly improved . Conclusion Huaihuai isoflavones have significant protective effect on estrogen-induced liver injury in mice.