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目的观察中药复方中不同剂量含碘中药(海藻、昆布)复方对实验性自身免疫性甲状腺炎(AIT)大鼠甲状腺自身抗体(TGAb、TPOAb)、凋亡调控蛋白Fas、FasL、Bcl-2、Bax的影响,并筛选中药复方中含碘中药的最佳剂量。方法取SD雌性大鼠75只,随机分为正常对照组,模型组,含碘中药复方低、中、高剂量组。酶联免疫法检测各组大鼠甲状腺抗体水平;免疫组化法检测各组大鼠甲状腺组织凋亡调控蛋白Fas、FasL和Bcl-2、Bax的变化。结果含碘中药复方各剂量组与模型组比较,甲状腺自身抗体水平均降低,甲状腺组织Fas、FasL、Bax的表达均减少,Bcl-2的表达增多,差异均有统计学意义(P<0.01);含碘中药复方中、高剂量组与低剂量组比较,差异有统计学意义(P<0.05),但中、高剂量组组间比较无明显差异(P>0.05)。结论含碘中药复方能够通过降低甲状腺自身抗体水平,抑制凋亡调控蛋白Fas、FasL、Bax的表达,增加Bcl-2的表达,起到治疗AIT的作用。
Objective To observe the effects of different doses of iodine-containing traditional Chinese medicine (seaweed and kelp) compound on the thyroid autoantibodies (TGAb, TPOAb), Fas, FasL, Bcl-2 and TGAb in experimental autoimmune thyroiditis (AIT) Bax, and screening the best dose of traditional Chinese medicine compound iodine Chinese medicine. Methods Seventy-five female SD rats were randomly divided into normal control group, model group and low-dose, middle-dose and high-dose iodine-containing traditional Chinese medicine compound prescriptions. Thyroid antibodies were detected by enzyme-linked immunosorbent assay (ELISA). The expressions of Fas, FasL, Bcl-2 and Bax in thyroid tissues were detected by immunohistochemistry. Results Compared with the model group, the levels of thyroid autoantibodies were decreased in all dosage groups containing iodine, the expressions of Fas, FasL and Bax decreased, while the expressions of Bcl-2 increased in thyroid tissue (P <0.01) (P <0.05). However, no significant difference was found between the medium and high dose groups (P> 0.05). The difference was statistically significant (P <0.05). Conclusion The compound prescription of iodine can play a role in the treatment of AIT by decreasing the level of thyroid autoantibodies, inhibiting the expression of apoptosis regulatory proteins Fas, FasL and Bax and increasing the expression of Bcl-2.