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目的：为探讨止血带休克发生过程中血管舒缩功能的改变及意义，以及与血管一氧化氮（ＮＯ）产生的关系。方法：采用Ｒｏｓｅｎｔｈａｌ方法复制大鼠止血带休克（ＴｏＳ）模型，分别测定对照组和ＴｏＳ组大鼠血浆中，主动脉孵育液中亚硝酸盐（ＮＯ－２）含量及主动脉组织中ｃＧＭＰ含量，一氧化氮合酶（ＮＯＳ）活性，同时观察了主动脉血管环舒缩功能的改变。结果：ＴｏＳ大鼠离体主动脉环对去甲肾上腺素的反应性降低；其血浆和主动脉孵育液中ＮＯ－２明显增加；主动脉ｃＧＭＰ含量增多；主动脉血管总ＮＯＳ活性加强，其中主要是诱导型ＮＯＳ（ｉＮＯＳ）活性增加。结论：ＴｏＳ大鼠主动脉ＮＯＳ活性加强，产生ＮＯ增多，造成血管低反应性。
Objective: To investigate the changes and significance of vasomotor function during tourniquet shock and its relationship with the production of nitric oxide (NO) in blood vessels. Methods: Rat tourniquet-induced shock (ToS) was induced by Rosenthal method. The content of nitrite (NO-2) and the content of cGMP in aortic tissue were measured in the plasma and aorta of control group and ToS group respectively. Nitric oxide synthase (NOS) activity, at the same time observed aortic vascular ring contraction function changes. Results: The reactivity of isolated aortic rings to norepinephrine was decreased in ToS rats. NO-2 in plasma and aorta was significantly increased, the content of cGMP in aorta was increased, and the activity of total NOS in aortas was enhanced Is an increase in inducible NOS (iNOS) activity. CONCLUSIONS: Too NOS activity in aortas of ToS rats is increased, resulting in increased NO and vascular hyporesponsiveness.