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【目的】从南海柳珊瑚共附生放线菌的次生代谢产物中寻找具有抗菌和抗附着活性的先导化合物。【方法】应用化学与生物活性相结合的筛选方法,从柳珊瑚共附生微生物中筛选获得代谢产物丰富且具有生物活性的目标菌株并通过大发酵提取浸膏,利用硅胶柱色谱、凝胶柱色谱和高效液相色谱等方法对发酵产物进行分离、纯化,运用波谱解析鉴定化合物的结构。【结果】从采自海南三亚的柳珊瑚(Muricella flexuosa)样品中分离到一株放线菌SCSGAA0009,鉴定为链霉属Streptomycessp.,从其改良ISP2发酵液中分离到新化合物N-(2-(1H-indol-3-yl)ethyl)propionamide(1)和已知化合物phenazine-1-carboxylic acid(2),其中化合物2对大肠杆菌和海洋细菌假单胞菌(Pseudoaltermonas piscida)具有较好抗菌活性,且有强抗草苔虫(Bugulaneritina)幼虫附着活性。【结论】首次从柳珊瑚共附生放线菌的次生代谢产物中获得新的生物碱化合物1,首次报道化合物2的抗海洋细菌活性和抗附着活性;从南海柳珊瑚共附生微生物的次生代谢产物中可以得到新化合物和活性化合物,这一来源的微生物资源值得深入研究。
【Objective】 The objective of this study is to find the lead compounds with antibacterial and anti-adhesion activities from the secondary metabolites of Actinomyces cobrans. 【Method】 The biologically active target metabolites were screened from the co-epiphytic microorganisms of Salix psammophila by chemical and biological methods. The target strains were extracted by large-scale fermentation and purified by silica gel column chromatography, Chromatography and high performance liquid chromatography and other methods of fermentation products were isolated and purified, the use of spectral analysis identified the structure of compounds. 【Result】 An actinomycete strain SCSGAA0009 was isolated from the sample of Muricella flexuosa collected from Sanya, Hainan Province and identified as Streptomyces. The new compound N- (2- (1) and phenazine-1-carboxylic acid (2), in which compound 2 has good antibacterial activity against Escherichia coli and Pseudoaltermonas piscida Activity, and strong anti-grass bug (Bugulaneritina) larvae attachment activity. 【Conclusion】 The new alkaloid compound 1 was obtained from the secondary metabolites of actinomycetes coexisted for the first time, and its anti-marine bacterial activity and anti-adhesion activity were reported for the first time. Secondary metabolites can be obtained in new compounds and active compounds, the source of microbial resources deserve further study.