本文通过克隆3’-N-去苯甲酰紫杉醇N-苯甲酰转移酶(3’-N-debenzoyltaxol Nbenzoyltransferase,DBTNBT)基因Dbtnbt,构建其表达载体p1303-SDbtnbtN,转化中国红豆杉细胞,经潮霉素抗性筛选获得转基因细胞系.转基因细胞分析结果表明,T-DNA及其包含的基因与宿主细胞染色体成功整合;转基因细胞中报告基因GusA-mgfp5正常表达;转基因细胞的Dbtnbt mRNA表达量是未转化细胞的1.33倍;转基因细胞的紫杉醇产量约为27.3μg/g,是未转化细胞的1.37倍.本研究结果表明,过表达Dbtnbt基因将中国红豆杉细胞的紫杉醇产量提高约37%. In this paper, we cloned the Dbtnbt 3’-N-debenzoyltaxol Nbenzoyltransferase (DBTNBT) gene and constructed its expression vector p1303-SDbtnbtN to transform Taxus chinensis cells. The results of transgenic cell analysis showed that T-DNA and its contained genes were successfully integrated with the host cell chromosomes, the gene GusA-mgfp5 was normally expressed in transgenic cells, and the expression of Dbtnbt mRNA in transgenic cells was 1.33 times that of untransformed cells, and the yield of paclitaxel in transgenic cells was about 27.3 μg / g, which was 1.37 times of that in untransformed cells.The results of this study showed that over-expression of Dbtnbt gene increased paclitaxel production by about 37% in Taxus chinensis cells.