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玉米籽粒形成期的库活性是弱势粒败育或灌浆受限的核心限制因子,明确弱势粒中内源激素及多胺水平对其库活性的调控机制,对探索密植条件下玉米弱势粒调控途径具有重要意义。本研究以典型玉米杂交种郑单958和先玉335为材料,在控制授粉条件下(不完全授粉Ic P、完全授粉CP),比较分析了成功发育弱势粒(Ic P处理)和发育不良弱势粒(CP处理)的内源激素及多胺水平差异及其与库活性的关系。结果表明,品种和年度对籽粒库活性、内源激素和多胺水平整体无显著影响。Ic P处理下弱势粒的可溶性酸性蔗糖转化酶(SAI)活性显著高于CP处理,平均差异和最大差异分别达13.5%和21.8%。在玉米籽粒形成期,弱势粒中玉米素和玉米素核苷(Z+ZR)、生长素(IAA)、赤霉素(GA3)和脱落酸(ABA)含量在两种控制授粉处理间无显著差异。弱势粒中多胺含量表现为Ic P处理显著高于CP处理,而乙烯释放速率则恰恰相反。弱势粒中SAI活性与多胺含量显著正相关,而与乙烯释放速率显著负相关,且多胺含量与乙烯释放速率显著负相关。可见,在玉米籽粒形成期,其弱势粒中Z+ZR、IAA、GA3和ABA与其库活性即SAI活性无关;弱势粒库活性主要受多胺和乙烯含量影响,多胺促进SAI活性而乙烯则抑制其活性,二者的平衡关系决定了弱势粒成功发育与否;多胺和乙烯平衡关系受同化物质供应水平的调控。
The activity of maize kernel during the grain-forming phase is the core restriction factor of weak-grain abortion or grouting limitation. The regulation mechanism of endogenous hormones and polyamines on the repertoire activity of weak grains is clarified. It is of great significance. In this study, typical maize hybrids Zhengdan 958 and Xiangyu 335 were used as materials to control the pollination conditions (incompletely pollinated Ic P and complete pollination CP). The results showed that the successful development of inferior grains (Ic P treatment) and dysplasia The difference of endogenous hormones and polyamines levels in granule (CP) and their relationship with the activity of the library. The results showed that variety and annual had no significant effect on the activity of grain bank, endogenous hormones and polyamine levels as a whole. The soluble acid sucrose invertase (SAI) activity of inferior grains under Ic P treatment was significantly higher than that of CP treatment with the average difference and maximum difference of 13.5% and 21.8% respectively. The content of zeatin, zeatin IAA, gibberellin GA3 and abscisic acid (ABA) in inferior grains was not significantly different between the two control pollination treatments during the grain-forming stage of maize difference. The content of polyamines in inferior grains showed that the Ic P treatment was significantly higher than the CP treatment, while the ethylene release rate was the opposite. There was a significant positive correlation between SAI activity and polyamine content in inferior grains, but negatively correlated with ethylene release rate, and there was a significant negative correlation between SAI activity and ethylene release rate. It can be seen that Z + ZR, IAA, GA3 and ABA in the weakened grains had no correlation with its library activity, ie, SAI activity during the grain-forming stage of maize. The activity of the weakened grain bank was mainly affected by polyamine and ethylene content. Polyamine promoted SAI activity while ethylene Inhibition of its activity, the balance between the two determines the successful development of inferior grains; polyamine and ethylene balance relationship regulated by the level of assimilation supplies.