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目的考察中药动脉栓塞微球的肝肿瘤动脉栓塞、细胞凋亡、坏死,肝功能变化,免疫及增敏作用。方法在透视下经连接管缓慢注入白兔肝肿瘤供血动脉,采用原位缺口末端标记法检测细胞的凋亡指数,免疫组织化学法检测肿瘤细胞中Bax和Bcl-2蛋白的表达,考察肿瘤细胞凋亡率、坏死率,肝功能变化及氯喹增敏作用;以小鼠碳粒廓清法研究免疫调节作用。结果中药栓塞微球中、高剂量组肿瘤生长抑制率与空白A组相比有显著差异(P<0.01);细胞凋亡指数低、中剂量组与A组相比有显著差异(P<0.01),与不含氯喹B组相比,差异显著(P<0.05);与低、中、高剂量组相比动物的ALT和AST值均显著低于B组(P<0.05);中药栓塞剂高、中剂量组廓清指数K值明显升高,吞噬指数α值也有一定增高(P<0.05)。结论实验提示中药栓塞微球具有形成肝肿瘤动脉栓塞,诱导肝癌细胞凋亡、坏死,保肝,增敏效应及免疫调节作用。
Objective To investigate the effects of arterial embolism microspheres on arterial embolism, apoptosis, necrosis, changes of liver function, immunity and sensitization in liver tumors. Methods The hepatic tumor of the hepatic artery was slowly infused through the connecting tube under fluoroscopy. The apoptosis index was detected by in situ nick end labeling. The expression of Bax and Bcl-2 in the tumor cells was detected by immunohistochemistry. Apoptotic rate, necrosis rate, changes of liver function and chloroquine sensitizing effect. The immunomodulatory effect of carbon clearance in mice was studied. Results The inhibitory rates of tumor growth in the medium and high dose groups of Chinese medicine embolism microspheres were significantly different from those in the blank group A (P <0.01), and the apoptotic index was lower in the medium dose group than in the A group (P <0.01) ), Compared with those in the group without chloroquine (P <0.05), ALT and AST in the animals were significantly lower than those in the low, medium and high dose groups (P <0.05) The values of K in the high and middle dose groups were significantly increased, and the phagocytic index α was also increased (P <0.05). Conclusion The experimental results suggest that the embolism microspheres of traditional Chinese medicine have the formation of arterial embolism in liver tumors and induce the apoptosis, necrosis, hepatoprotection, sensitization and immunomodulatory effects of hepatocellular carcinoma cells.