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为了节约微藻生物柴油的生产成本,用沼液作为小球藻生长的培养基,可以得到微藻油脂,还可以净化沼液,从而起到了防治环境污染的作用。研究小球藻在不同浓度沼液(25%、50%、75%和100%沼液浓度)中生长和油脂积累情况,结果表明小球藻在各低浓度沼液(25%、50%沼液浓度)中能很好地生长,延滞期较短,而在高浓度沼液(75%、100%沼液浓度)中,延滞期较长,并且50%浓度沼液培养的小球藻总油脂含量最高。因此选取50%浓度沼液作为小球藻的基本培养基,研究了在50%浓度沼液中,小球藻在不同接种量、光照强度、光暗周期、初始pH条件下的生长和油脂积累情况。得出最适小球藻生长和油脂积累的条件是接种OD值1.023左右、光强4 000 lx、光暗周期20∶4、初始pH值为7.0。
In order to save the production cost of microalgae biodiesel, biogas slurry can be used as culture medium for the growth of chlorella, and the microalgae oil and fat can be obtained, and the biogas slurry can also be purified, thereby playing a role of preventing and controlling environmental pollution. The growth and lipid accumulation of Chlorella sp. In different concentrations of biogas slurry (25%, 50%, 75% and 100% biogas slurry concentration) was studied. The results showed that Chlorella sp. Liquid concentration), the lag period is short, while in the high concentration biogas slurry (75%, 100% biogas slurry concentration), the lag period is longer, and the chlorella culture medium with 50% Grease content is the highest. Therefore, 50% biogas slurry was selected as the basic medium for Chlorella to study the growth and lipid accumulation of Chlorella under different inoculum size, light intensity, light and dark cycle, initial pH in 50% Happening. The optimal conditions for the growth and lipid accumulation of Chlorella were OD of about 1.023, light intensity of 4000 lx, light and dark cycle of 20: 4 and initial pH of 7.0.