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目的 了解北京地区 6岁以下儿童中急性下呼吸道感染 (ALRI)患儿的病毒感染状况 ;探讨人类偏肺病毒 (hMPV)感染在儿童ALRI中的作用及所致支气管肺炎的临床特征。方法 (1)采集 2 0 0 1年 2月至 2 0 0 3年 3月就诊于首都儿科研究所 4 4 6例年龄 1个月至 5岁的ALRI住院患儿的鼻咽分泌物 ,同时应用间接免疫荧光 (IFA)及病毒分离方法进行人类呼吸道合胞病毒 (hRSV)、流感病毒 (甲、乙型 )、副流感病毒 (Ⅰ、Ⅱ、Ⅲ型 )及腺病毒等 7种常见呼吸道病毒的病原检测。 (2 )采用逆转录聚合酶链反应 (RT PCR)方法对 2 0 0 2年 11月至 2 0 0 3年 3月住我院内科的ALRI患儿中经 7种呼吸道常见病毒病原检测阴性的 2 16份鼻咽洗液 ,进行了hMPV基因检测 ,并分析hMPV感染患儿的临床资料。结果 (1) 4 4 6例患儿中 7种常见呼吸道病毒总阳性例数为 2 0 9例 (46 86 % ) ,hRSV阳性者最多为 16 3例 (36 5 5 % )。 (2 )在 2 16份上述 7种呼吸道病原检测阴性的标本中 6 9份测到hMPV阳性扩增产物 (31 9% ) ,占同期送检标本的 19 17%。结论 (1)hRSV是北京地区冬春季节婴幼儿ALRI的主要病原。 (2 )2 0 0 2年 11月至 2 0 0 3年 3月我院ALRI住院患儿中有 19 17%与hMPV感染有关
Objective To investigate the status of viral infection in children with acute lower respiratory tract infection (ALRI) in children under 6 years of age in Beijing and to explore the role of human metapneumovirus (hMPV) infection in children with ALRI and the clinical features of bronchopneumonia. Methods (1) To collect nasopharyngeal secretions from 466 children aged 1 month to 5 years who were hospitalized in ALRI between February 2001 and March 2003 in Capital Institute of Pediatrics. At the same time, Indirect immunofluorescence (IFA) and virus isolation methods were used to detect seven common respiratory viruses of human respiratory syncytial virus (hRSV), influenza virus (A and B), parainfluenza virus (Ⅰ, Ⅱ and Ⅲ) and adenovirus Pathogen detection. (2) Reverse transcriptase polymerase chain reaction (RT PCR) was used to detect seven common respiratory virus pathogens in children with ALRI from November 2002 to March 2003 in our hospital 2 16 nasopharyngeal washes, hMPV gene detection, and analysis of hMPV infection in children with clinical data. Results (1) The total number of positive cases of 7 kinds of common respiratory viruses among 446 children was 209 (46.86%), and the highest number of hRSV positive cases was 163 (36.55%). (2) The hMPV-positive amplification products (31.9%) were detected in 6 of 16 samples negative for the above-mentioned 7 kinds of respiratory pathogens, accounting for 19 17% of the samples examined in the same period. Conclusions (1) hRSV is the main pathogen of ALRI in infants and young children in winter and spring in Beijing. (2) Between November 2002 and March 2003, 19 17% of children hospitalized with ALRI in our hospital were associated with hMPV infection