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目的分析通过荧光定量PCR检测乙肝病毒DNA(HBVDNA)载量以及采用磁微粒化学发光法定量检测乙型肝炎表面抗原(HBs Ag)之间的关系。方法选取我院2015年6月—2017年5月收治的118例门诊及住院肝炎患者血清标本,分别采用荧光定量PCR以及ECLIA检测标本的HBV-DNA载量、HBs Ag含量,分析两种检测结果。结果 118例标本中,66例同时检测HBs Ag、HBV-DNA阳性,占55.9%。HBV-DNA载量和HBs Ag定量之间并无明显相关性。结论荧光定量PCR检测HBV-DNA载量以及采用磁微粒化学发光法定量检测HBs Ag之间并没有明显相关性,两者联合检测可为临床诊治提供科学、合理的依据。
Objective To analyze the relationship between HBV DNA load (HBVDNA) and quantitative detection of hepatitis B surface antigen (HBsAg) by fluorescence quantitative PCR. Methods A total of 118 serum samples from outpatients and hospitalized patients with hepatitis were collected from June 2015 to May 2017 in our hospital. HBV-DNA and HBsAg levels were detected by fluorescence quantitative PCR and ECLIA respectively. . Results Of 118 cases, 66 cases were detected HBsAg at the same time, HBV-DNA positive, accounting for 55.9%. There was no significant correlation between HBV-DNA load and HBsAg quantitation. Conclusion There is no significant correlation between the detection of HBV-DNA by fluorescence quantitative PCR and the quantitative detection of HBsAg by magnetic particle chemiluminescence. The combined detection of HBV and DNA can provide a scientific and reasonable basis for clinical diagnosis and treatment.