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应用抗ｐ５３蛋白的单克隆抗体对３１例上皮性卵巢癌新鲜组织冰冻切片的ｐ５３蛋白表达行ＡＢＣ法免疫组化测定。结果显示，ｐ５３蛋白阳性部位主要位于癌细胞核内，３例标本（９．７％）伴有细胞浆内轻度阳性染色，３１例标本阳性２３例，阳性率为７４．２％。ｐ５３蛋白的过度表达与组织学分级、临床分期有关（Ｐ＜０．０５），与组织学类型无关（Ｐ＞０．０５）。说明ｐ５３基因的突变可发生在上皮性卵巢癌的早期、中期和晚期，并呈逐渐增加趋势，与肿瘤的生长、扩散关系密切。测定ｐ５３蛋白有助于确定肿瘤的分化程度和临床分期。
Immunohistochemical ABC method was used to detect the expression of p53 protein in fresh frozen sections of 31 cases of epithelial ovarian cancer using monoclonal antibody against p53 protein. The results showed that the positive part of p53 protein was mainly located in the nucleus of the cancer cells. In 3 specimens (9.7%) with slight positive staining in cytoplasm, 23 cases were positive in 31 cases, the positive rate was 74.2%. Overexpression of p53 protein was correlated with histological grade and clinical stage (P <0.05), but not with histological type (P> 0.05). These results suggest that the mutation of p53 gene may occur in the early, middle and late stages of epithelial ovarian cancer and gradually increase, closely related to tumor growth and spread. Determination of p53 protein helps to determine the degree of tumor differentiation and clinical stage.