突变体［Ｂ１８Ｉｌｅ］猪胰岛素前体经分离纯化、转肽，得到重组［Ｂ１８Ｉｌｅ］人胰岛素。［Ｂ１８Ｉｌｅ］人胰岛素能结晶，其与受体的结合能力为猪胰岛素的８２％，保留了与猪胰岛素基本相同的体内生物活力。从本文结果和分析表明Ｂ１８Ｖａｌ可能不是胰岛素表现生物功能所必需的。 Mutant [B18Ile] porcine insulin precursor was isolated and purified to transpeptide to obtain recombinant [B18Ile] human insulin. [B18Ile] Human insulin crystallizes and binds to the receptor at 82% of porcine insulin, retaining essentially the same in vivo biological activity as porcine insulin. From the results and analysis of this paper shows that B18Val may not be necessary for the performance of insulin biological function.