论文部分内容阅读
目的 探讨吗啡对钳夹损伤坐骨神经后其传入纤维终末在脊髓Ⅱ板层分布的影响。 方法 用显示抗氟化物酸性磷酸酶 (FRAP)组织化学方法 ,借助图像分析仪测量损伤坐骨神经后 15d和 30d吗啡组和对照组大鼠脊髓Ⅱ板层FRAP阳性反应物面积。 结果 损伤坐骨神经后吗啡组和对照组大鼠脊髓Ⅱ板层FRAP阳性反应区均有不同程度的缺失 ;对照组损伤坐骨神经后 30d的FRAP阳性反应物面积比 15d的FRAP阳性反应物面积增大 40 %。吗啡组损伤坐骨神经后 30d的FRAP阳性反应物面积比 15d的FRAP阳性反应物面积增大 2 2 %;同时也比对照组损伤坐骨神经后 30d的FRAP阳性反应物面积增大 19%。 结论 随着损伤坐骨神经后大鼠存活时间延长 ,其脊髓Ⅱ板层FRAP阳性反应物面积呈恢复性增大 ;吗啡能使坐骨神经损伤大鼠脊髓Ⅱ板层的FRAP阳性反应物面积明显增大。
Objective To investigate the effects of morphine on the distribution of spinal lamina Ⅱ after the injured sciatic nerve is clamped. Methods FRAP histochemical method was used to measure the area of FRAP-positive reactants in spinal cord II of morphine group and control group on the 15th day and the 30th day after sciatic nerve injury with image analyzer. Results FRAP positive reaction zone of spinal cord lamina Ⅱ in the injured sciatic nerve group was significantly different from that in the control group. In the control group, the FRAP positive reaction area of the sciatic nerve increased by 40% . The area of FRAP-positive reactant in morphine group increased by 22% compared with that in 15d after 30 days sciatic nerve injury, and the area of FRAP-positive reactant increased by 19% 30 days after sciatic nerve injury in the control group. Conclusions With the prolonged survival of rats after sciatic nerve injury, the area of FRAP-positive reactant in the platelet of spinal cord II is recovered and increased. Morphine can significantly increase the area of FRAP-positive reactant in the spinal cord II of sciatic nerve injury rats.