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目的:探讨Girdin蛋白在调控小鼠受精卵早期发育微丝聚集中的作用。方法:采用免疫荧光染色观察Girdin蛋白和F-actin在小鼠受精卵中的共定位情况。激光共聚焦显微镜观察Girdin表达敲低后小鼠受精卵分裂形态及分裂率。结果:在小鼠受精卵中存在Girdin蛋白并且Girdin蛋白与F-actin存在共定位。敲低Girdin蛋白的表达,小鼠受精卵出现不规则分裂,10μmol/L Girdin si RNA处理组小鼠受精卵有28.93%到达2-细胞期,而注射20μmol/L Girdin si RNA组小鼠受精卵只有15.1%到达2-细胞期。并且Girdin表达敲低的受精卵微丝不能正确聚集。结论:Girdin蛋白在调控小鼠受精卵早期分裂微丝聚集中发挥作用。
Objective: To investigate the role of Girdin protein in the regulation of microfilament aggregation during early development of mouse fertilized eggs. Methods: Immunofluorescence staining was used to observe the co-localization of Girdin protein and F-actin in mouse zygotes. Laser scanning confocal microscopy was used to observe the morphology and rate of division of fertilized eggs in mice after Girdin knockdown. Results: The Girdin protein was present in the mouse zygotes and the Girdin protein co-localized with F-actin. Knocking down the expression of Girdin protein, the mouse zygotes showed irregular division. The zygotes of Girdin si RNA-treated mice reached 28.53% in 2-cell stage, while the 20 μg / Only 15.1% reached the 2-cell stage. And Girdin expression knock-down fertilized egg microfilament can not accumulate correctly. Conclusion: The Girdin protein plays a role in the regulation of early microchidosis in mouse fertilized eggs.