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目的:探讨红花注射液对内毒素性急性肝损伤(AHI)大鼠模型的保护作用。方法:以小剂量脂多糖/D-氨基半乳糖(LPS/D-GalN)造成大鼠AHI模型,使用红花注射液进行干预,并设还原型谷胱甘肽(GSH)注射液为阳性对照组;分别于6、24和48h取血和肝组织,测定血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)和一氧化氮(NO)的水平变化,以及肝组织超氧化物歧化酶(SOD)活力和脂质过氧化产物丙二醛(MDA)的水平。结果:模型组血清ALT、AST、NO及肝组织MDA的水平明均明显高于空白对照组(P均<0.01),肝组织SOD活力明显低于空白对照组(P<0.01)。红花治疗组各时间点血清ALT、AST、NO及肝组织MDA的水平明显低于模型组(P<0.05或P<0.01),肝组织SOD活力明显高于模型组(P<0.01)。红花治疗组和阳性对照组之间比较无明显差异(P>0.05)。结论:红花注射液能够降低内毒素性AHI大鼠的氧自由基水平,抑制脂质过氧化反应,对AHI有较好的防治作用。
Objective: To investigate the protective effect of safflower injection on endotoxic acute liver injury (AHI) in rats. Methods: The rat AHI model was induced by low dose lipopolysaccharide / D-galactosamine (LPS / D-GalN), and then treated with safflower injection. GSH was also used as positive control The levels of serum ALT, AST and NO were measured at 6, 24 and 48 hours after operation, and the changes of liver tissue superoxide Dismutase (SOD) activity and lipid peroxidation product malondialdehyde (MDA) levels. Results: The levels of serum ALT, AST, NO and MDA in the model group were significantly higher than those in the blank control group (all P <0.01). The activity of SOD in liver tissue was significantly lower than that in the blank control group (P <0.01). The levels of serum ALT, AST, NO and liver MDA at different time points in safflower treatment group were significantly lower than those in model group (P <0.05 or P <0.01). SOD activity in liver tissue was significantly higher than that in model group (P <0.01). There was no significant difference between safflower treatment group and positive control group (P> 0.05). Conclusion: Safflower injection can reduce the levels of oxygen free radicals in endotoxin-induced AHI rats, inhibit lipid peroxidation, and have a good preventive and therapeutic effect on AHI.