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目的:观察凋亡诱导蛋白(Fas)及凋亡执行蛋白(Caspase-3)在多囊肝病中的表达及临床意义。方法:对43例多囊肝病患者囊壁肝组织和周围正常肝组织分别采用TUNEL观察标本细胞凋亡,免疫组织化学方法检测凋亡诱导蛋白Fas,凋亡抑制蛋白Bcl-2,凋亡执行蛋白Caspase-3表达并结合临床资料进行分析。结果:TUNEL显示囊肿壁肝细胞的凋亡指数(AI)明显高于正常组织;Fas和Caspase-3在囊肿壁肝细胞中的表达明显高于周围正常组织;Bcl-2在囊肿壁肝细胞中的表达明显低于周围正常组织。相关性分析表明,囊肿壁肝细胞AI与Fas和Caspase-3的表达呈正相关;与Bcl-2的表达呈负相关。临床相关分析表明,TUNEL表达阳性与否和年龄、性别、家族史、合并肝外囊肿、症状、血清白蛋白和谷丙转氨酶(GPT)水平无关;Fas的表达与合并肝外囊肿有关,但与年龄、性别、家族史、症状、血清白蛋白和GPT水平无关。结论:肝细胞凋亡在多囊肝病的发生发展中起重要作用。Fas的表达对于判断多囊肝病人是否合并肝外囊肿具有重要的指导意义。
OBJECTIVE: To observe the expression and clinical significance of apoptosis-inducing protein (Fas) and caspase-3 in patients with polycystic liver disease. Methods: Thirty-three patients with polycystic liver disease were enrolled in this study. TUNEL was used to observe the apoptosis of cystic liver tissue and surrounding normal liver tissue. The expressions of apoptosis-inducing protein Fas, apoptosis-inhibitory protein Bcl-2, Caspase-3 expression and clinical data analysis. Results: TUNEL showed that the apoptotic index (AI) of cyst wall hepatocytes was significantly higher than that of normal tissues. The expression of Fas and Caspase-3 in cyst wall hepatocytes was significantly higher than that of normal surrounding tissues. Bcl-2 expression in cyst wall hepatocytes The expression was significantly lower than the surrounding normal tissue. Correlation analysis showed that the AI of cyst wall was positively correlated with the expression of Fas and Caspase-3, but negatively correlated with the expression of Bcl-2. Clinical correlation analysis showed that TUNEL expression was not associated with age, gender, family history, extrahepatic cyst, symptoms, serum albumin and alanine aminotransferase (GPT) levels; Fas expression was associated with extrahepatic cyst, Age, gender, family history, symptoms, serum albumin, and GPT levels were not related. Conclusion: Hepatocyte apoptosis plays an important role in the occurrence and development of polycystic liver disease. Fas expression for the judgment of patients with polycystic liver extrahepatic cyst has an important guiding significance.