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目的:研究麝香、冰片、安息香和苏合香四味芳香开窍药对脑缺血再灌注损伤大鼠的保护作用机制。方法:线栓法制备局灶性脑缺血再灌注损伤大鼠模型,观察药物对大鼠术前、缺血2h再灌注4h、10h、22h时体温的影响;比色法测定大鼠缺血2h再灌注22h后血清NO含量与NOS活性;ELISA法测定缺血侧脑组织中IL-1β和IL-6含量。结果:冰片(0.2g/kg)能明显抑制大鼠缺血2h再灌注4h、10h、22h时体温的异常升高,麝香(66.6mg/kg)和苏合香(1.332g/kg)仅在再灌注后4h时明显抑制体温升高,安息香(1.0g/kg)作用不明显。麝香、冰片和苏合香能显著降低模型动物血清NO含量,麝香、冰片还能显著抑制血清NOS活性。冰片、麝香能显著降低缺血侧脑组织IL-1β、IL-6含量,苏合香和安息香的作用不明显。结论:芳香开窍药对脑缺血再灌注损伤大鼠有一定的保护作用,其中麝香、冰片作用为优,其次苏合香,安息香作用较弱,作用机制可能与抑制炎症介质释放减轻炎症损伤及抗NO神经毒性有关。冰片对模型动物体温升高的良好抑制作用,从一个侧面反映了该药区别于其他三味药物的“寒凉”之性。
Objective: To study the protective mechanism of Musk, Borneol, Benzoin and Su Hexiang aromatic resuscitation drugs on cerebral ischemia-reperfusion injury in rats. Methods: The rat model of focal cerebral ischemia-reperfusion injury was established by thread occlusion. The effects of drugs on the body temperature of rats preoperatively, 4h, 10h, 22h after reperfusion were observed. Serum NO and NOS activities were measured at 2 hours and 24 hours after reperfusion. The levels of IL-1β and IL-6 in ischemic brain tissue were detected by ELISA. RESULTS: Borneol (0.2g / kg) significantly inhibited the abnormal increase of body temperature at 4h, 10h, 22h after reperfusion in rats at 2h after ischemia. Musk (66.6mg / kg) After 4h significantly inhibited body temperature, benzoin (1.0g / kg) effect is not obvious. Musk, Borneol and Su Hexiang can significantly reduce the serum NO content of model animals, musk, borneol can significantly inhibit serum NOS activity. Borneol and musk can significantly reduce the content of IL-1β and IL-6 in ischemic brain tissue, and the effect of Su-a-xiang and benzoin is not obvious. CONCLUSION: Aromatic Reopening Agent has certain protective effect on rats with cerebral ischemia-reperfusion injury. Musk and borneol are the best, followed by weak synergistic effect of benzalkonium and benzoin. The mechanism may be related to inhibiting the release of inflammatory mediators and reducing inflammation and NO Neurotoxicity related. The excellent inhibitory effect of borneol on the rise of body temperature in model animals reflects the “cold” nature of the drug, which is different from other three-flavor drugs.