论文部分内容阅读
为探讨血小板活化因子（ＰＡＦ）对肾小球系膜细胞（ＧＭＣ）产生活性氧的调节作用，本研究观察了ＰＡＦ对体外培养的大鼠ＧＭＣ产生超氧化物阴离子（Ｏ）和过氧化氢（Ｈ－２Ｏ－２）的影响。结果表明１０￣（－９）Ｍ／Ｌ的ＰＡＦ已能诱导ＧＭＣ产生Ｏ和Ｈ２Ｏ２（１．１４±０．４２ｎｍｏｌ／１０￣５ＧＭＣ、０．９７±０．１６ｎｍｏｌ／１０－５ＧＭＣ），这一效应呈剂量依赖性；溶ＰＡＦ无诱导ＧＭＣ产生活性氧作用；特异性ＰＡＦ受体拮抗剂ＢＮ５２０２１抑制ＰＡＦ的刺激作用，抑制作用也呈剂量依赖性，５×１０￣（－５）Ｍ／ＬＢＮ５２０２１完全抑制ＧＭＣ合成活性氧。本研究说明ＰＡＦ可诱导ＧＭＣ合成活性氧。
In order to investigate the regulatory effect of platelet activating factor (PAF) on reactive oxygen species in glomerular mesangial cells (GMCs), the effect of PAF on the production of superoxide anion (O) and hydrogen peroxide H-2O-2). The results showed that 10 ~ (-9) M / L PAF could induce GMC to produce O and H2O2 (1.14 ± 0.42nmol / 10 ~ 5GMC, 0.97 ± 0.16nmol / 10-5GMC) In a dose-dependent manner. PAF did not induce reactive oxygen species (ROS) -induced GMC production. The specific PAF receptor antagonist BN52021 inhibited PAF stimulation in a dose-dependent manner. 5 × 10 -5 M / LBN52021 completely inhibited GMC synthesis of reactive oxygen species. This study shows that PAF can induce GMC synthesis of reactive oxygen species.