论文部分内容阅读
用快速SPA-ELISA和SPA-ELISA一步法检测囊虫病人的滤纸血滴片和血清,前者阳性率分别为83.05%和86.44%.后者分别为96.67%和94.91%,均无统计学差异(P>0.05);两法的假阳性率均为3.33%。实验条件的研究表明:血滤纸4℃浸泡过夜和室温浸泡2h的阳性率为83.05%和84.38%,直径1.2cm和0.6cm阳性率为83.05%和85.29%,亦均无显著性差异(P>0.05)。说明滤纸血滴片可以代替血清样本,具有敏感、安全、简便以及非常实用等优点,血滴片SPA-ELISA在囊虫病的诊断及流行病学调查中有较好的应用价值。
A one-step rapid SPA-ELISA and SPA-ELISA for the detection of cysticercosis patients filter paper blood serum and serum, the former positive rates were 83.05% and 86.44%. The latter were 96.67% and 94.91%, respectively, with no significant difference (P> 0.05). The false positive rates of both methods were 3.33%. The experimental results show that the positive rates of blood filter paper immersed at 4 ℃ for 2h and at room temperature for 2h were 83.05% and 84.38%, respectively. The positive rates of diameter 1.2cm and 0.6cm were 83.05% and 85.29% , There was no significant difference (P> 0.05). It shows that the filter paper blood droplet can replace the serum sample, which is sensitive, safe, simple and practical. The SPA-ELISA has good application value in the diagnosis and epidemiological investigation of cysticercosis.