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目的了解2003—2010年山东省食源性沙门菌的血清型分布及耐药情况,为制定政策提供科学依据。方法根据GB/T 4789—2008《食品卫生微生物学检验》方法进行沙门菌菌种鉴定和血清分型;采用临床实验室标准研究所(CLSI)推荐的微量肉汤稀释法进行药物敏感性试验和结果判读,确定耐药表型。结果 120株确定血清型沙门菌分属5个群(8个亚群)24个血清型,肠炎和德比沙门菌为最常见血清型。胥瓦增格隆、维也纳、布里丹、明斯特、乌赫利等9种血清型为2010年首次检出血清型。126株沙门菌对14种抗生素均有不同程度的耐药,萘啶酮酸的耐药率最高(61.90%),其次为四环素(33.33%)、氨苄青霉素(31.75%)、磺胺嘧啶(26.19%);而敏感性以头孢吡肟最高(5.37%),其次是阿米卡星(9.52%)、环丙沙星(10.32%)。结论山东省食源性沙门菌耐药形势严峻,应加强畜牧养殖业中抗生素使用的管理,从食物链的源头和动物食品生产过程中采取合理的干预措施,保障食品安全。
Objective To understand the serotype distribution and drug resistance of food-borne Salmonella in Shandong Province during 2003-2010 and to provide a scientific basis for formulating policies. Methods According to GB / T 4789-2008 “Microbiological examination of food hygiene” method, Salmonella species identification and serotyping were carried out. Drug susceptibility test was conducted by using CLSI microdilution method Interpretation of the results to determine the drug resistance phenotype. Results 120 serotypes of Salmonella belonged to 5 serogroups (8 subgroups) and 24 serotypes, enteritis and Salmonella deserei were the most common serotypes. Schwarzwald, Vienna, Buridan, Münster, Uheli and other nine serotypes for the first time in 2010 serotypes. 126 strains of Salmonella resistant to 14 kinds of antibiotics, with the highest rate of resistant to naringin (61.90%), followed by tetracycline (33.33%), ampicillin (31.75%) and sulfadiazine (26.19% ) And the highest sensitivity was cefepime (5.37%), followed by amikacin (9.52%) and ciprofloxacin (10.32%). Conclusion The situation of drug-resistant Salmonella resistance in Shandong Province is severe. Management of antibiotic use in livestock husbandry should be strengthened. Food safety should be ensured by taking reasonable intervention measures from the source of the food chain and animal food production.