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目的 应用微量渗透泵 (Mini- Osmotic pump,MOP)给正常动物耳蜗和庆大霉素 (GM) )致聋豚鼠耳蜗长期恒速灌注人工外淋巴和表皮生长因子 (Epidermal growth factor,EGF) ,观察其对听力和内耳组织形态的影响。方法 用 Alzet model- 2 0 0 4型 MOP向三组各 1 0只的正常豚鼠 A组和 GM致聋豚鼠 B组 ,耳蜗底回鼓阶灌注人工外淋巴液 ,同法给 GM致聋豚鼠耳蜗灌注EGF设为 C组 ,在 MOP埋植前和埋植后 1、2、3和 4周测定 ABR反应阈值 ,用扫描电镜观察各组动物耳蜗 Corti器的组织形态改变。结果 MOP给正常动物灌注人工外淋巴对 ABR和耳蜗内外毛细胞无明显影响 ,而给 GM致聋组豚鼠耳蜗灌注 EGF可促进受损内耳组织细胞修复 ,使ABR阈值改善。结论 应用 MOP可向耳蜗长期恒速灌注人工外淋巴 ,对耳蜗功能和形态无明显影响 ;而长期恒速灌注 EGF对 GM耳中毒豚鼠的耳蜗毛细胞有修复治疗作用。可使 ABR反应阈值下降
Objective To observe the long-term constant perfusion of artificial peripheral lymphatic and epidermal growth factor (EGF) in guinea pig cochlea induced by normal animal cochlea and gentamicin (GM) by using Mini-Osmotic pump (MOP) Its effect on hearing and inner ear tissue morphology. Methods The Alzet model - 2004 MOP was used to infiltrate the cochlear to the cochlea of guinea pigs with GM The perfusion EGF was set as group C, and the threshold of ABR reaction was measured before MOP implants and 1, 2, 3 and 4 weeks after implantation. The morphological changes of cochlear Corti were observed by scanning electron microscopy. RESULTS: Perioperative perfusion of artificial lymphocytes by MOP had no significant effect on ABR and inner and outer hair cells of the cochlea. However, perfusion of EGF with cochlear of GM deafness group promoted repair of injured inner ear cells and improved ABR threshold. CONCLUSION: MOP can prolong constant infusion of artificial perilymph to the cochlea without any effect on the function and morphology of the cochlea. Long-term constant infusion of EGF can repair the cochlear hair cells of guinea pigs with GM ototoxicity. ABR response threshold can be reduced