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蛋白酶-抗蛋白酶平衡失调在COPD发病中起重要作用。弹性蛋白酶(ELT)活性增高,可能是上述失衡的主要机制。寻找对ELT有抑制作用的药物,特别是从祖国中草药中发掘对防治COPD具有重要意义。山豆根、连翘、甘草等中药分别经水煎,乙醇提取,干燥,最后加水稀释成1ml含生药2.0g备用;常规制备琼脂糖板(含1%琼脂,0.08弹性蛋白,0.02%SDS及0.05%NaN3),打孔,每孔加样20μl,置37℃温育24小时,测量各孔溶解环直径,反应酶
The imbalance of protease-antiprotease plays an important role in the pathogenesis of COPD. Elevated elastase (ELT) activity may be the main mechanism for the above imbalances. Looking for drugs that have inhibitory effects on ELT, especially from the Chinese herbal medicine in the motherland, is of great significance for the prevention and treatment of COPD. Shandougen, forsythia, licorice and other traditional Chinese medicines were decocted, extracted with ethanol, dried, and finally diluted with water to prepare 1ml of crude drug 2.0g. The conventional preparation of agarose plates (containing 1% agar, 0.08 elastin, 0.02% SDS and 0.05% NaN3), punched, add 20μl per well, incubate at 37°C for 24 hours, measure the diameter of dissolving rings in each well, reaction enzyme