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目的 :探讨NF κBI(NF κB抑制剂 )与融合基因PSP94 TNFαD11a联合注射抗前列腺癌的作用。方法 :制作PC 3细胞裸鼠动物模型。肌注PSP94与TNFαD11a融合基因的 pcDNA PSP94 TNFαD11a真核表达质粒DNA ,并联合肌注NF κBI ,同时设 pcDNA PSP94、pcDNA PSP94联用NF κBI、pcDNA PSP94 TNFαD11a、NF κBI、空载体、生理盐水和环磷酰胺对照组。注射后第 10天处死动物 ,称瘤重、计算抑瘤率。结果 :pcDNA PSP94 TNFαD11a联合NF κBI用药组抑瘤率 (36 % )高于 pcDNA PSP94 TNFαD11a组 (2 0 % ) ,前者瘤重〔(1 87± 0 .934) g〕明显低于后者〔(2 .32± 1.373) g〕。差异具有显著性 (P <0 .0 5)。结论 :NF κBI可提高TNF的抗前列腺癌作用。
Objective: To investigate the effect of NF κB inhibitor (NF κB inhibitor) combined with the fusion gene PSP94 TNFαD11a on anti-prostate cancer. Methods: The animal model of PC 3 cell nude mice was made. PcDNA PSP94 TNFαD11a fusion gene was intramuscularly injected into pcDNA PSP94 TNFαD11a plasmid DNA, and combined with intramuscular injection of NF κBI, at the same time set pcDNA PSP94, pcDNA PSP94 combined with NF κBI, pcDNA PSP94 TNFαD11a, NF κBI, empty vector, normal saline and loop Phosphamide control group. The animals were sacrificed on the 10th day after injection and weighed to calculate the tumor inhibition rate. RESULTS: The tumor inhibition rate (36%) of pcDNA3.094 TNFαD11a combined with NF κBI group was significantly higher than that of pcDNA-PSP94 TNFαD11a group (20%), the former was significantly lower than the latter [(1887 ± 0. 934) g 2 .32 ± 1.373) g]. The difference was significant (P <0.05). Conclusion: NF κBI can enhance the anti-prostate cancer effect of TNF.