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目的 探讨13 2 Ge对矽肺血清SOD活性、LPO水平及肝Kupffer细胞功能的影响。方法 将实验大鼠分为A、B、C三组 ,A、B两组气管内注入石英尘混悬液 ,C组注入生理盐水 ;A组饮用含13 2 Ge水 ,B、C两组饮用自来水。 6个月后取心脏血 ,用亚硝酸盐形成法测定血清SOD同工酶活性 ,硫代巴比妥酸比色法测定血清LPO水平。用肝Kupffer细胞诱生肿瘤坏死因子 (TNF)试验测定矽肺鼠细胞免疫功能。结果 A组大鼠血清总SOD和CuZnSOD活性与B组相比显著增高 (P <0 0 1) ,A组血清LPO水平 ( 6 17± 1 2 7)与B组 ( 8 3 1± 1 11)相比明显降低 (P <0 0 1)。B组肝Kupffer细胞诱生TNF的能力略有下降 ,但A组则略有上升。结论 13 2 Ge可能具有减轻由矽尘引起的大鼠巨噬细胞损伤并对免疫功能低下有一定改善的作用
Objective To investigate the effects of 13 2 Ge on SOD activity, LPO level and liver Kupffer cell function in silicosis serum. Methods The experimental rats were divided into three groups: A, B and C, groups A and B were injected with tracheal suspension of trachea and group C with normal saline. Group A was drinking with 13 2 Ge water and groups B and C Tap water. After 6 months, the blood samples were collected for determination of serum SOD isoenzyme activity by nitrite formation method and serum LPO level by thiobarbituric acid colorimetry. Tumor necrosis factor (TNF) test was induced by Kupffer cells in liver to determine cellular immune function in silico mice. Results The levels of total serum SOD and CuZnSOD in group A were significantly higher than those in group B (P <0.01). The serum levels of LPO in group A (6 17 ± 1 27) and group B (83 11 ± 1 11) Compared with the significant decrease (P <0 0 1). The ability of hepatic Kupffer cells in group B to induce TNF decreased slightly, but that in group A increased slightly. Conclusions 13 2 Ge may reduce the damage of rat macrophages induced by silica dust and improve the immune function