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目的 :研究不同浓度的一氧化氮 (nitricoxide,NO)对食管癌细胞核DNA损伤作用的特征性规律。方法 :以硝普钠 (sodi umnitroprusside ,SNP)作为NO的体外供体 ,以食营癌细胞系EC10 9作为细胞模型 ,采用单细胞凝胶电泳法检测不同浓度的SNP作用一定时间后 ,食管癌细胞核DNA被损伤的情况。结果 :无论SNP的作用时间是 8h ,还是 16h ,彗星状细胞核所占的百分率均越来越高 ,经 χ2 检验差异有显著性意义 (P <0 .0 1)。曲线回归分析结果表明 ,食管癌细胞彗星状细胞核生成的百分率与SNP的浓度之间明显相关。结论 :在一定的条件下 ,NO对食管癌细胞核DNA的损伤作用具有明显的浓度依赖性特征
Objective: To study the characteristic regularity of DNA damage of esophageal cancer cells with different concentrations of nitric oxide (NO). Methods: Sodi umnitroprusside (SNP) was used as the in vitro donor of NO and EC109 was used as a cell model. Single cell gel electrophoresis was used to detect the effect of different concentrations of SNP on esophageal carcinoma Nuclear DNA is damaged. Results: The percentage of comet nuclei was higher than that of SNP for 8h or 16h. There was significant difference between the two groups (P <0.01). Curve regression analysis showed that there was a significant correlation between the percentage of comet nucleated cells and the concentration of SNP in esophageal cancer cells. CONCLUSION: Under certain conditions, NO has a significant concentration-dependent effect on DNA damage of esophageal cancer cells