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目的 研究大网膜脂肪组织中 L eptin基因的表达及在肥胖和胰岛素抵抗发病机制中的作用。 方法 选择肥胖患者 (BMI(30 kg/m2 )和正常人 (BMI<2 5 kg/m2 )各 10例 ,抽提大网膜脂肪组织 RNA;用半定量 RT-PCR方法研究肥胖合并胰岛素抵抗患者和正常人大网膜脂肪组织中 L eptin基因的表达。 结果 实验组 L eptinm RNA水平显著高于对照组 (P<0 .0 1)。 结论 肥胖患者体内可能存在 L eptin抵抗。
Objective To study the expression of L eptin gene in omental adipose tissue and its role in the pathogenesis of obesity and insulin resistance. Methods 10 cases of obese patients (BMI (30 kg / m2) and normal persons (BMI <25 kg / m2) were selected and the omentum adipose tissue RNA was extracted. Semiquantitative RT-PCR was used to study the relationship between obesity and insulin resistance And normal human omental adipose tissue.Results The level of L eptinm RNA in the experimental group was significantly higher than that in the control group (P0.01) .Conclusion L eptin resistance may exist in obese patients.