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目的：观察ＥＧｂ及其所含成分槲皮素和内酯Ｂ对谷氨酸诱导的神经元损伤的拮抗作用．方法：对小鼠下丘脑弓状核作形态学观察和图象分析，采用ＭＴＴ法检测皮层原代培养神经元的活性，用Ｆｕｒａ２测定单个神经元内游离钙离子的浓度（［Ｃａ２＋］ｉ）．结果：ＥＧｂ及其内酯Ｂ能够保护神经元对抗谷氨酸的损伤，阻止谷氨酸诱发的［Ｃａ２＋］ｉ的升高．ＥＧｂ能够逆转谷氨酸诱导的下丘脑弓状核神经元核面积的减少．结论：ＥＧｂ及其内酯Ｂ可以通过降低［Ｃａ２＋］ｉ水平对抗谷氨酸神经毒性，内酯Ｂ很可能是ＥＧｂ中对抗谷氨酸神经毒性的主要成分之一．
OBJECTIVE: To observe the antagonism of glutamate-induced neuronal damage by EGb and its constituents quercetin and lactone B. METHODS: Morphology and image analysis of the hypothalamic arcuate nucleus were performed. MTT assay was used to detect the activity of primary cortical neurons. Fura 2 was used to determine the concentration of intracellular free calcium in individual neurons ([Ca2+]. i). RESULTS: EGb and its lactone B could protect neurons against glutamate and prevent glutamate-induced [Ca2+]i elevation. EGb can reverse the glutamate-induced decrease in the nuclear area of the hypothalamic arcuate nucleus. CONCLUSION: EGb and its lactone B can reduce glutamate neurotoxicity by decreasing the level of [Ca 2+] i. Lactone B is probably one of the main components of EGb against glutamate neurotoxicity.