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目的:优选金樱子蜜炙工艺,建立蜜炙金樱子乙酸乙酯部位的指纹图谱,为该药材的炮制工艺标准化和质量控制提供参考。方法:以总多糖、总黄酮和浸出物含量的综合评分为指标,采用U7(76)均匀设计考察加蜜量、炒制温度、炒制时间和稀释用水量对金樱子蜜炙工艺的影响。采用《中药色谱指纹图谱相似度评价系统》2004B版软件进行指纹图谱分析,流动相乙腈(A)-0.1%磷酸水溶液(B)梯度洗脱(0~20 min,5%~9%A;20~30 min,9%~15%A;30~35 min,15%~17%A;35~60 min,17%~27%A;60~75 min,27%~30%A;75~90 min,30%~40%A),检测波长360 nm。结果:最佳蜜炙工艺为加药材量20%炼蜜,用水稀释炼蜜使稀释用水/炼蜜量0.4,炒制温度180℃,炒制时间6 min。10批次蜜炙金樱子乙酸乙酯部位指纹图谱共标定出7个共有峰,各批次样品的指纹图谱相似度均>0.80。结论:采用多指标综合加权评分法优选的金樱子蜜炙工艺稳定可行,建立的指纹图谱研究方法简单、准确、重复性好,可用于蜜炙金樱子的质量控制。
OBJECTIVE: To optimize the fingerprinting of Rosa laevigata and to establish the fingerprinting map of the ethyl acetate part of Rosa roxburghii. It can provide reference for the processing standardization and quality control of the Rosa roxburghii radix. Methods: The total score of total polysaccharides, total flavonoids and extract content as an indicator, using U7 (76) uniform design to investigate the amount of honey, frying temperature, the time of the system and the dilution of water on the Rosa nigrum sunburn process . Fingerprinting analysis was carried out using software 2004B of Chinese Medicines Chromatographic Fingerprint Similarity Evaluation System. The mobile phase consisted of gradient elution of acetonitrile (A) -0.1% phosphoric acid (B) (0-20 min, 5-9% A; 20 30 min, 9% to 15% A, 30 to 35 min, 15% to 17% A, 35 to 60 min, 17% to 27% A, 60 to 75 min, 27% to 30% A, 75 to 90 min, 30% ~ 40% A), detection wavelength of 360 nm. Results: The best honeyburn process was adding 20% honey refining material, refining honey diluted with water to dilute the water / refining honey volume 0.4, the firing temperature 180 ℃, the firing time 6 min. Seven batches of common peaks were obtained from 10 batches of fingerprints of Rosa laevigata ethyl acetate. The similarity of fingerprints of batches of samples was> 0.80. CONCLUSION: The Rosa roxburghii Nourishing Sunburn, which is optimized by the multi-index comprehensive weighted score method, is stable and feasible. The established fingerprinting method is simple, accurate and reproducible. It can be used for the quality control of Rosa roxburghii.